Propanoic acid, 2,2-dimethyl-, 1,1'-[6-[10-[bis(1-methylethyl)amino]-13-cyano-1-oxo-9,11-dioxa-2-aza-10-phosphatridec-1-yl]-2',4,7-trichloro-3-oxo-7'-phenylspiro[isobenzofuran-1(3H),9'-[9H]xanthene]-3',6'-diyl] ester

Description

VIC is an asymmetrical xanthene dye (two flanking rings are not the same). This dye is used for the design of qPCR probes. The spectral properties of VIC are similar to HEX and JOE.

This phosphoramidite reagent allows to introduce the label onto 5'-terminus of the oligonucleotide.

Coupling: 10 minutes.

Deprotection: standard conditions with ammonium hydroxide; AMA (solution of 30% ammonium hydroxide/40% aqueous methylamine 1:1 v/v) (15 minutes at 65°C) can be used with minor side product forming.

Uses

VIC phosphoramidite, 6-isomer is a VIC derivative that can be used for conjugating VIC to other molecules. VIC can be used for labeling oligonucleotides at the 5’-end[1].

References

[1] de Kok JB, et al. Rapid genotyping of single nucleotide polymorphisms using novel minor groove binding DNA oligonucleotides (MGB probes). Hum Mutat. 2002 May;19(5):554-9. DOI:10.1002/humu.10076

Abs/Em Maxima

525/546 nm

Fluoresene quantum yield

0.53

Extinction Coefficient

103000

CF260

0.07

CF280

0.07