Uses
Bromocresol Green sodium salt is used as a pH indicator. It is a pH sensitive triphenylmethane dye useful in various colorimetric detection studies and as an activity tracking dye for DNA agarose gel electrophoresis. It is also used in TLC (thin-layer chromatography) for visualizing compounds containing functional groups having pKa less than 5.
Biological Activity
Bromocresol Green is mainly used for measuring serum albumin. In presence of acidic conditions, bromocresol green interacts with albumin, thereby resulting in an increase in absorbance at 630nm. The change in absorbance is linked with the concentration of albumin. Bromocresol Green is also a pH indicator, changing from yellow (pH 4.0) to blue color (5.4).
