Description
Phallacidin is a natural mycotoxin first isolated from the death cap mushroom,
A. phalloides. It binds filamentous actin (F-actin) like phalloidin , but contains a carboxyl group for coupling reactions.
Uses
Phallacidin is a natural mycotoxin first isolated from the death cap mushroom. It binds filamentous actin like phalloidin.
Uses
Toxin Phallacidin is a weak fluorescent bicyclic peptide probe in aquous solution, Which is isolated from Amanita phalloides mushroom and could be used for blocking F-actin staining by labeled phallotoxins, which promotes actin polymerization.
Definition
ChEBI: A homodetic bicyclic heptapeptide having a sulfide bridge.
Biochem/physiol Actions
Phallacidin, which is a bicyclic toxin from the Amanita phalloides mushroom, has been shown to inhibit F-actin degradation by proteolytic enzymes including trypsin and α-chymotrypsin.
Enzyme inhibitor
This cyclic heptapeptide toxin (FWfree-acid = 846.92 g/mol; CAS 26645-35-2) from the poisonous green fungus Amanita phalloides stabilizes actin filaments and inhibits actin filament depolymerization. NBD phallacidin is a high-affinity, filamentous actin probe consisting of rabbit actin that is conjugated by reacting its thiol group with 4-chloro-7- nitrobenzofurazan, a yellow-green fluorescent dye. NBD phallacidin (Excitation at 465 nm; Emission at 536 nm) selectively stains F-actin un a manner that is superior to antibody staining, preferably in fixed and permeabilized samples. See Phalloidin
