Description
1,10-Phenanthroline (18851-33-7) is a high affinity chelator of divalent metal ions and may be used to inhibit zinc metalloenzymes.1? It is a potent inhibitor of JAMM-type isopeptidases (DUBs) acting via chelation of an active site Zn2+.? It may be used when preparing cell lysates for enrichment of ubiquitinated proteins? which are especially sensitive to DUBs in the JAMM family.2,3
Chemical Properties
White fine crystalline powder
Uses
1,10-Phenanthroline monohydrochloride monohydrate is used as an analytical reagent for colorimetric determination of iron.
References
1) Swann?et al?(1981)?Zinc metalloenzyme properties of active and latent collagenase from rabbit bone;. Biochem.J.,?195?41
2) Cooper?et al. (2009) K63-specific deubiquitination by two JAMM/MPN+ complexes: BRISC-associated Brcc36 and proteasomal Poh1; EMBO J.,?28?621
3) Lu?et al. (2015)?Substrate degradation by the proteasome: A single-molecule kinetic analysis; Science,?348?200
