Description
Coelestin Blue B 40, C.I. 51 050 [1562-90
9] is correspondingly obtained by action of 4
nitrosodiethylaniline hydrochloride with gallamide.
Chemical Properties
Black powder
Uses
Celestine Blue is a cationic dye used in cell staining. Dyes and metabolites.
Uses
To dye fabrics a navy-blue color with faint reddish tinge. As nuclear and connective tissue stain. One of the ingredients of Picro-Mallory stain: Lendrum, McFarlane, J. Pathol. Bacteriol. 50, 381 (1940).
Definition
ChEBI: Celestin blue B is an organic chloride salt composed of 1-carbamoyl-7-(diethylamino)-3,4-dihydroxyphenoxazin-5-ium and chloride ions in a 1:1 ratio. A histological dye used in solution with an iron alum mordant as a hematoxylin substitute in the H&E stain. It has a role as a fluorochrome and a histological dye. It contains a Celestin blue B(1+).
Preparation
excessive amounts of ?N,N-diethyl-4-nitrosobenzenamine or N,N-diethyl-4-(phenyldiazenyl)benzenamine?and 3,4,5-Trihydroxybenzamide reaction.
Synthesis
In an enamelled 6000 L ket tle equipped with a wooden gate stirrer, 110 kg of gallamide and 2000 L of alcohol (denatured, 94 %) are brought to a rapid boil. Over 10 h, 240 kg of nitrosodiethylaniline hydrochloride, dissolved in 1500 L of ethanol, is allowed to flow in. After addition of 21 kg of potassium carbonate, the reaction mixture is boiled for an other 1 h and allowed to cool to 20 ℃; 40 L of 20 % hydrochloric acid is added, and the mix ture is pressed into asuctionfilter withafiltering stone bottom. After stirring with 500 L of 20 % hydrochloric acid, the mixture is filtered under suction until the product is well dried, and the product is further dried at 70 ℃. Yield: 95 %. The ethanol is recovered by distillation.
Properties and Applications
red light navy blue. Soluble in water for red light purple, slightly soluble in ethanol for blue. In concentrated sulfuric acid for Daisy blue, dilution after magenta. Dye aqueous solution and sodium hydroxide as blue purple and blue precipitation.
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Standard
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Ironing Fastness
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Light Fastness
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Fulling
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Persperation Fastness
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Soaping
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Water
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Alkali
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Acid
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ISO
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5
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5
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3-4
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5
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4
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3
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