Chemical Properties
green crystalline powder
Uses
Azure B is used for staining semi-thin sections of plant tissue. This dye is also useful in the NCCLS method for blood smears and Lillie's modified Nocht's method for paraffin sections.
Uses
Used for staining reticulocytes. Azure B is used for preparing azure eosin stains for blood smears.
Definition
ChEBI: An organic chloride salt having 3-(dimethylamino)-7-(methylamino)phenothiazin-5-ium as the counterion. It is used in making azure eosin stains for blood smear staining and is an active metabolite of methylene blue.
Biochem/physiol Actions
Azure B is a cationic dye. It is part of the Romanowsky stain, which is used for staining of blood films. It is also used for the localization of nucleic acids and lignin.
Purification Methods
Azure B has been twice recrystallised from H2O and dried at 100o/1hour in an oven. [Beilstein 27 III/IV 5151.]
