Uses
α1-Acid glycoprotein from human plasma has been used:
- as negatively charged protein to investigate the adsorption processes onto the surface of polyelectrolyte multilayers
- to incubate plates coated with purified antiserum in the study to investigate its serum concentration by ELISA
- to study the effect of its different concentrations on the antiviral activity of human immunodeficiency virus (HIV) protease inhibitors
Uses
GLYCOPROTEIN, ALPHA1-ACID HUMAN was used to study nonviral gene delivery to the lung with copolymer-protected and transferrin-modified polyethylenimine.
General Description
α-1-Acid glycoprotein (AGP)/orosomucoid (ORM), a glycoprotein has a molecular weight of 41-43kDa. It is considered as one of the important acute phase proteins in humans and rats. AGP may belong to the lipocalin family.
Biochem/physiol Actions
α1-Acid glycoprotein possesses immunomodulating effects. It has the ability to bind to acidic drugs, like phenobarbital.
Purification Methods
Purify the glycoprotein 1 acid by passage through a carboxymethyl cellulose column, then through a Sephadex G-25 column. [Aronson et al. J Biol Chem 243 4564 1968.]
