Description
L-γ-Glutamyl-p-nitroanilide (67953-08-6; GPNA) is a substrate for γ-glutamyl transpeptidase. GPNA is used for the determination of γ-glutamyl transpeptidase hydrolytic activity via spectrophotometric monitoring of p-nitroaniline released by the action of the enzyme on GPNA.1 GPNA has also been shown to be an inhibitor of glutamine uptake – solute-linked carrier family A1 member 5 (SLC1A5).2Cellular uptake of L-glutamine (regulated by SLC1A5) is a rate-limiting step that activates mTOR and is, thus, an interesting cancer chemotherapy target.3
References
[1] COOPER A J, GRIFFITH O. γ‐Glutamyl Transpeptidase[C]. 2002: 0. DOI:
10.1002/0471203076.emm0514[2] C. SEAN ESSLINGER Joseph F R Kimberly A Cybulski. Nγ-Aryl glutamine analogues as probes of the ASCT2 neutral amino acid transporter binding site[J]. Bioorganic & Medicinal Chemistry, 2005, 13 4: Pages 1111-1118. DOI:
10.1016/j.bmc.2004.11.028[3] PAUL NICKLIN. Bidirectional transport of amino acids regulates mTOR and autophagy.[J]. Cell, 2009: 521-534. DOI:
10.1016/j.cell.2008.11.044
