Description
5α-Cholestane is a sterol that has been found in dust samples from urban and rural paved and agricultural and public unpaved roads. It has been used as an internal standard for the quantification of phytosterols by HPLC-MS/MS and fecal sterols by GC-FID and GC-MS.
Chemical Properties
WHITE CRYSTALLINE POWDER
Uses
5α-Cholestane was used as standard in cholesterol analysis by GC and HPLC.
Uses
α-Cholestane is the trans-decalin homolog of Coprostane.
Definition
ChEBI: The 5alpha-stereoisomer of cholestane.
Biochem/physiol Actions
5α-Cholestane is a sterol produced endogenously from cholesterol and has been isolated from human feces. It is derived from cholesterol by the action of intestinal microorganisms. Derivatives of 5α-cholestane in plants are called Brassinosteroids that selectively activate the PPI3K/Akt pathway.
Purification Methods
Crystallise 5-cholestane from Et2O/EtOH or EtOAc. [Ruzicka et al. Helv Chim Acta 16 327 1933, Beilstein 5 III 1132, 5 IV 1227.]
