Uses
Because of its unusual recognition sequence,
Sfi I is useful for analyzing and cloning large DNA fragments.
Note: The octameric recognition sequence of
Sfi I rarely occurs in the genomes of many organisms, especially those that have low G+C content.
General Description
Sfi I recognizes the sequence GG°C*CNNNN↓NGG°C°C and generates fragments with 3′-cohesive termini.
Note: Sfi I sites are a subset of
Bgl I sites. The cleavage position in the N5 region is identical.
