91930-79-9

Because of its unusual recognition sequence, Sfi I is useful for analyzing and cloning large DNA fragments.
Note: The octameric recognition sequence of Sfi I rarely occurs in the genomes of many organisms, especially those that have low G+C content.

Sfi I recognizes the sequence GG°C*CNNNN↓NGG°C°C and generates fragments with 3′-cohesive termini.
Note: Sfi I sites are a subset of Bgl I sites. The cleavage position in the N5 region is identical.