Preparation
179 mg of MnCl2 was added to 2.85 L of Tris-HCl (50 mM) solution to adjust the pH to 8.0, and the temperature was maintained at 37 °C. 143 mg of NAD+ pyrophosphatase from *E. coli* (EcNADD) and 10 g of NADH were added to the solution, and the mixture was stirred for 2 hours. The NMNH solution was obtained by preparative chromatography, the pH was adjusted to 10 with NaOH, and the solution was concentrated under reduced pressure to obtain 3.8 g of β-nicotinamide mononucleotide, reduced form, disodium salt(NMNH) solid.
Description
NMNH (reduced Nicotinamide Mononucleotide) is a new potent NAD+ precursor. It is a new form of NMN, can increase NAD levels to a much higher extent.
NMNH is a fluorescent molecule, in contrast to NMN. The molecular formulae for NMNH is C11H17N2O8P and it is also known as 1-(5-O-phosphono-beta-D-ribofuranosyl) -1,4-dihydropyridine-3-carboxamide Reduced nicotinamide mononucleotide.
Uses
β-Nicotinamide mononucleotide (NMNH), reduced form disodium (β-NMN) is the oxidized form of NAD+ precursor and is a NAD+ enhancer. β-Nicotinamide mononucleotide, reduced form disodium can be reduced to dihydronicotinamide mononucleotide (NMNH). NMNH inhibits glycolysis, TCA cycle, and cell growth[1].
General Description
β-Nicotinamide mononucleotide, reduced form disodium (β-NMN) is the
oxidized form of NAD+ precursor and is a NAD+ enhancer. β-Nicotinamide
mononucleotide, reduced form disodium can be reduced to
dihydronicotinamide mononucleotide (NMNH). NMNH inhibits glycolysis, TCA
cycle, and cell growth.
References
[1] Liu Y, et al. Reduced Nicotinamide Mononucleotide (NMNH) Potently Enhances NAD+ and Suppresses Glycolysis, the TCA Cycle, and Cell Growth. J Proteome Res. 2021 May 7;20(5):2596-2606. DOI:
10.1021/acs.jproteome.0c01037
