ABTS

Chemical Properties

Light green powder

Uses

Diammonium-2,2’Azino-bis(3-ethylbenzothiazoline-6-sulfonate) has been shown to inhibit catalase, a mammalian antioxidant enzyme which degrades hydrogen peroxide into water and oxygen species.

Uses

• 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt, ABTS, has been used as a substrate in ELISA (enzyme-linked immunosorbent assay).
• ABTS is also used to measure glucose concentrations in different solutions including blood serum.
• The food industry uses ABTS to measure the antioxidant capacities within different foods.
• Since it is less readily oxidized than TMB and OPD substrates, ABTS is less sensitive for ELISA applications. Thus ABTS has some advantageous over TMB and OPD in cases of large background results
• It is recommended for ELISA (microwell) procedures, not recommended for membrane applications.

Uses

Chromogenic substrate for peroxidase assays.2,2'-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) diammonium salt is widely utilized as a peroxidase substrate suitable for use in enzyme-linked immunosorbent assay (ELISA) procedures. It plays an important role in the food industry to measure the antioxidant capacities of foods. It is used to observe the reaction kinetics of specific enzymes and involved in glucose estimating reagent in blood serum.

General Description

ABTS, 2,2′-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid), is water soluble chemical compound used as chromogenic substrate:

• ABTS together with hydrogen peroxide for reaction with peroxidase enzymes (like HRP for example).
• ABTS together with laccase or bilirubin oxidase.

ABTS mainly used in ELISA (enzyme-linked immunosorbent assay) procedures.
A peroxidase reaction of ABTS in the presence of hydrogen peroxide produces a green soluble end product which can be read spectrophotometrically at 405nm. The reaction may be stopped with 1% sodium dodecyl sulfate (SDS). ABTS, 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt is light sensitive.