Asunaprevir

Gefahreninformationscode (GHS)

• Bildanzeige (GHS)

  

• Alarmwort

  Warnung

• Gefahrenhinweise

  H302：Gesundheitsschädlich bei Verschlucken.

  H315：Verursacht Hautreizungen.

  H319：Verursacht schwere Augenreizung.

  H335：Kann die Atemwege reizen.

• Sicherheit

  P261：Einatmen von Staub vermeiden.

  P305+P351+P338：BEI KONTAKT MIT DEN AUGEN: Einige Minuten lang behutsam mit Wasser spülen. Eventuell vorhandene Kontaktlinsen nach Möglichkeit entfernen. Weiter spülen.

Asunaprevir Chemische Eigenschaften,Einsatz,Produktion Methoden

• Beschreibung

  Sold under the trade name Sunvepra®, asunaprevir received approval in Japan as part of a combination treatment for the hepatitis C virus (HCV). Working in concert with daclatasvir (IX) (vide infra), asunaprevir is a unique treatment for HCV, as it is free from both interferon and ribavirin and is administered orally. This direct-acting anti-viral, which was developed by Bristol–Myers Squibb (BMS), works as an NS3/4A protease inhibitor, representing a valuable treatment option for patient populations who are unable to receive, or do not respond to, the standard course of treatment—peginterferon/ribavirin.

• Beschreibung

  Asunaprevir is an inhibitor of the hepatitis C non-structural 3/4A serine protease, which is required for viral replication. It is a direct-acting inhibitor of hepatitis C virus (HCV). It is commonly used in combination with daclatasvir and beclabuvir, which are also direct-acting HCV inhibitors.

• Verwenden

  Asunaprevir is an inhibitor of the viral enzyme serine protease HCV NS3. Also functions as a second generation NS3/4A protease inhibitor used in the treatment of hepatitis C.

• Definition

  ChEBI: Asunaprevir is an oligopeptide.

• Synthese

  The preparation of chloroquinoline 27 began with bromination of commercially available acetophenone 20, which was carefully carried out on over 1 kg scale in a reactor equipped with an HBr scrubbing mechanism (bubbler outfitted) to furnish a-bromoketone 21. Next, nucleophilic displacement of the bromide using sodium diformamide (22) under phase transfer conditions led to the putative intermediate 23. This system underwent in situ deprotection of the diformamide functionality followed by cyclization under mildly acidic conditions to arrive at isoquinolone 24 in 78% yield across the two-step, one-pot sequence. Next, methylation of the hydroxyl group at C-4 was carried out using methanolic methanesulfonic acid at elevated temperatures followed by treatment with aqueous ammonium hydroxide to quench any excess acid. Subsequent treatment with phosphorous oxychloride furnished dichloroisoquinoline 26 in good yield. A nucleophilic aromatic substitution reaction employing potassium t-butoxide was used to establish an arylpyrrolidino ether linkage and, upon workup with mildly acidic conditions, the proline derivative 27 emerged in 59% yield.
  
  Although the synthesis of asunaprevir vinyl cyclopropane subunit 35 has been described by researchers at BMS, it is interesting to note that this subunit, or structural derivatives of 35, have been found within a number of other antiviral drugs, particularly those which target inhibition of NS3 protease as its mechanism of action. For example, researchers at Boehringer-Ingelheim have described a scale synthesis of (1R,2S)-1-amino-2-vinylcyclopropane carboxylic acid (des-N-tert-butoxycarbonyl (Boc) 32; also referred to as ??vinyl-ACCA?ˉ) as a means to incorporate this subunit into that company?ˉs antiviral agent BILN 2061. A macrocyclic version of this same cyclopropane-containing system is found in the antiviral agents paritaprevir hydrate (XXVII) developed by Enanta and Abbvie, and a saturated version in vaniprevir (XXXVII) developed by Merck and described later in this review (vide infra). The preparation of 32 described by Beaulieu and co-workers was performed on pilot-plant scale and the details surrounding the conversion of 32 to 35 are described by the BMS patent. Beginning with commercially available methyl glycine HCl (28), condensation with benzaldehyde in the presence of a dehydrating reagent (trimethyl orthoformate) and base led to the transient imine 29. This underwent a highly diastereoselective alkylation reaction whereby treatment with lithium t-butoxide followed by subjection to 1,4- dibromo-2-butene facilitated an SN2¨CSN20 reaction. Subsequent acidification and Boc protection resulted in 30-rac as a racemate that existed as a single diastereomer having the vinyl and the ester groups in a cis configuration. The authors postulate that the lithium enolate intermediate which forms upon the initial alkylation binds the proximal bromine atom which accounts for the stereoselectivity of the reaction. Next, selective saponification of the undesired enantiomer 31b took place via treatment with acalase 2.4 L, which is a remarkably versatile and relatively inexpensive enzyme capable of resolving a wide variety of racemic esters,49 under basic conditions. This enzymatic resolution returned a 49% yield of the desired ester 31a (theoretical 50%), and a simple aqueous workup removed the undesired acid 31b. Methyl ester 31a was then saponified using methanolic lithium hydroxide to give 32, which was subsequently coupled with cyclopropanesulfonamide 33 to deliver 34 in excellent yield. Next, removal of the Boc group using TFA followed by salt formation with ethereal HCl delivered the key cyclopropane subunit 35.
  Enzyminhibitor
  This highly selective HCV antiviral (FW = 748.29 g/mol; CAS 630420-16- 5; Symbol: ASV), also named BMS-650032, targets NS3 protease, a serine proteinase required for Hepatitis C Virus (HCV) polyprotein processing, showing good antiviral activity against replicons based on HCV Genotype 1a (EC50 = 4 nM), Genotype 1b (EC50 = 1.2 nM), Genotype 4 (EC50 = 1.8 nM), Genotype 5 (EC50 = 1.7 nM), and Genotype 6 (EC50 = 0.9 nM). It is far less effective against Genotype 2 (EC50 = 67 nM) and Genotype 3 (EC50 = >1100 nM). Asunaprevir is a peptidomimetic that occupies the active site, inhibiting the HCV NS3/4A serine protease, with little or no action against related viruses. The average Ki for ASV is approximately 0.4 nM and 0.2 nM for Genotype 1a and Genotype 1b, respectively. Its acylsulfonamide moeity interacts noncovalently with the NS3 protease – unlike telaprevir’s α-ketoamide moiety, which is linked covalently to NS3/4A’s catalytic serine and reverses slowly over time. ASV exhibits an excellent selectivity index (>40,000x) against all serine/cysteine proteases evaluated, including human leukocyte elastase, porcine pancreatic elastase, and three members of the chymotrypsin family. Although ASV shows a low barrier to resistance, it can be combined with daclatasvir to achieve a very high rate of viral eradication in both na?ve and treatment-experienced patients, showing a sustained virological response rate of 80%-90%.

• Lager

  Store at -20°C

• Einzelnachweise

  [1] scola pm, sun lq, wang ax, chen j, sin n, venables bl, sit sy, chen y, cocuzza a, bilder dm, d'andrea sv, zheng b, hewawasam p, tu y, friborg j, falk p, hernandez d, levine s, chen c, yu f, sheaffer ak, zhai g, barry d, knipe jo, han yh, schartman r, donoso m, mosure k, sinz mw, zvyaga t, good ac, rajamani r, kish k, tredup j, klei he, gao q, mueller l, colonno rj, grasela dm, adams sp, loy j, levesque pc, sun h, shi h, sun l, warner w, li d, zhu j, meanwell na, mcphee f. the discovery of asunaprevir (bms-650032), an orally efficacious ns3 protease inhibitor for the treatment of hepatitis c virus infection. j med chem. 2014 mar 13;57(5):1730-52.
  [2] mcphee f, sheaffer ak, friborg j, hernandez d, falk p, zhai g, levine s, chaniewski s, yu f, barry d, chen c, lee ms, mosure k, sun lq, sinz m, meanwell na, colonno rj, knipe j, scola p. preclinical profile and characterization of the hepatitis c virus ns3 protease inhibitor asunaprevir (bms-650032). antimicrob agents chemother. 2012 oct;56(10):5387-96.

Asunaprevir Upstream-Materialien And Downstream Produkte

Asunaprevir Anbieter Lieferant Produzent Hersteller Vertrieb Händler.

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