Oxidase, Ascorbat

Bezeichnung:Oxidase, Ascorbat

CAS-Nr9029-44-1

Englisch Name:ASCORBATE OXIDASE

CBNumberCB7221176

SummenformelN/A

Molgewicht0

MOL-DateiMol file

Synonyma

Oxidase, Ascorbat

Oxidase, Ascorbat physikalisch-chemischer Eigenschaften

storage temp.	2-8°C
Löslichkeit	Soluble in aqueous buffers.
Aggregatzustand	powder
Farbe	faintly bluish

Sicherheit

S-Sätze:	24/25
WGK Germany	3
F	10-21
HS Code	35079090

Gefahreninformationscode (GHS)

ASCORBATE OXIDASE Chemische Eigenschaften,Einsatz,Produktion Methoden

Chemische Eigenschaften
Appearance : Light blue amorphous powder, lyophilized
Stability : Store at －20℃ (A decrease in activity of ca.20% may occur at 5℃ within 6 months.)
Molecular weight : 132,000, 140,000
Isoelectric point : between 6.0 and 7.8 , 8.2
Michaelis constant : 2.5×10－4M (Ascorbate)
Structure : 8 copper atoms per mol of enzyme
Inhibitors : cyanide, Na2S, diethyldithiocarbamate (Na)
Optimum pH : 5.6
Optimum temperature : approx. 30℃
pH Stability : pH 7.0－10.0 (25℃, 17hr)
Thermal stability : below 40℃ (pH 8.0, 30min)
Substrate specificity : This enzyme oxidizes ascorbic acid and several ascorbic derivatives.
Verwenden
Biochemical research.
Verwenden
Ascorbate oxidase, from Cucurbita sp., may be used to study oxidative stress and heat stress response and tolerance. Also used in ascorbic acid assays to study the heat stress response of Arabidopsis and useful for enzymatic determination of ascorbic acid and for eliminating the interference of ascorbic acid in clinical analysis.
Definition
An enzyme found in plant tissue that acts upon ascorbic acid in the presence of oxygen to produce dehydroascorbic acid.
Allgemeine Beschreibung
Ascorbate oxidase is a homodimeric enzyme, which comprises 552 amino acid residues per subunit (zucchini). It corresponds to a molecular mass of 70kDa per subunit. This enzyme is mainly found in plants, fungi and eubacteria.
Biochem/physiol Actions
Ascorbate oxidase (AO) participates in cell growth by regulating reduction/oxidation (redox) of the apoplast. This enzyme can synthesize the oxidative molecule dehydroascorbate acid (DHA) in the apoplast. It is also involved in cell elongation and enlargement development.

Oxidase, Ascorbat Anbieter Lieferant Produzent Hersteller Vertrieb Händler.

9029-44-1, ASCORBATE OXIDASE Verwandte Suche:

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