5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt Struktur

5-Brom-4-chlor-3-indolylhydrogen-phosphat-p-toluidin (1:1)

Bezeichnung:5-Brom-4-chlor-3-indolylhydrogen-phosphat-p-toluidin (1:1)

CAS-Nr6578-06-9

Englisch Name:5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt

CBNumberCB4854083

SummenformelC8H6BrClNO4P.C7H9N

Molgewicht433.63

MOL-Datei6578-06-9.mol

Synonyma

5-Brom-4-chlor-3-indolylhydrogenphosphat - p-toluidin (1:1)

5-Brom-4-chlor-3-indolylhydrogen-phosphat-p-toluidin (1:1)

5-Brom-4-chlor-3-indolylhydrogen-phosphat-p-toluidin (1:1) physikalisch-chemischer Eigenschaften

Schmelzpunkt	194-195℃
storage temp.	-20°C
Löslichkeit	DMF: 20 mg/mL
Aggregatzustand	tablets
Farbe	White to Orange to Green
InChI	InChI=1S/C8H6BrClNO4P.C7H9N/c9-4-1-2-5-7(8(4)10)6(3-11-5)15-16(12,13)14;1-6-2-4-7(8)5-3-6/h1-3,11H,(H2,12,13,14);2-5H,8H2,1H3
InChIKey	QEIFSLUFHRCVQL-UHFFFAOYSA-N
SMILES	C12C(Cl)=C(C=CC=1NC=C2OP(=O)(O)O)Br.C1(C)C=CC(N)=CC=1
CAS Datenbank	6578-06-9(CAS DataBase Reference)
EPA chemische Informationen	1H-Indol-3-ol, 5-bromo-4-chloro-, dihydrogen phosphate (ester), compd. with 4-methylbenzenamine (1:1) (6578-06-9)

Sicherheit

Kennzeichnung gefährlicher	Xi
R-Sätze:	36/37/38-40
S-Sätze:	26
RIDADR	2811
WGK Germany	3
F	3-8-10
HazardClass	6.1
PackingGroup	III
HS Code	29339190

Gefahreninformationscode (GHS)

5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt Chemische Eigenschaften,Einsatz,Produktion Methoden

R-Sätze Betriebsanweisung:
R36/37/38:Reizt die Augen, die Atmungsorgane und die Haut.
R40:Verdacht auf krebserzeugende Wirkung.
S-Sätze Betriebsanweisung:
S26:Bei Berührung mit den Augen sofort gründlich mit Wasser abspülen und Arzt konsultieren.
Chemische Eigenschaften
White to light brown powder
Verwenden
For the colorimetric detection of alkaline phosphatase-labeled molecules, 5-Bromo-4-chloro-3-indolyl phosphate (BCIP) and Nitro Blue Tetrazolium (NBT) are available. The BCIP/NBT substrate system is versatile and functions in a variety of applications, including Northern Southern, and Western blotting, in situ hybridization, and immunohistochemistry.
BCIP is provided in two salt forms: the disodium salt which is soluble in water and the p-toluidine form which is soluble in dimethylformamide. These salt forms may be used to prepare a stock solution that in combination with NBT and a reaction buffer, form a substrate solution for alkaline phosphatase. This substrate system, when incubated with alkaline phosphatase, produces an insoluble NBT diformazan product that is easily observable with its purple color. For added convenience, a mixture of BCIP and NBT is provided in an easily dissolvable tablet form or as a ready-to-use liquid.
Allgemeine Beschreibung
BCIP serves as substrate for alkaline phosphatase. The reaction product has a blue color and is insoluble in water. The blue color can be visually detected.
Biochem/physiol Actions
5-bromo-4-chloro-3-indolyl phosphate (BCIP) can be used to visualize the phosphatase activity. It can be used as a substrate to monitor secreted embryonic alkaline phosphatase?activity in solution.

5-Brom-4-chlor-3-indolylhydrogen-phosphat-p-toluidin (1:1) Anbieter Lieferant Produzent Hersteller Vertrieb Händler.

6578-06-9, 5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt Verwandte Suche:

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