BCIP/NBT Struktur

BCIP/NBT

Bezeichnung:BCIP/NBT

CAS-Nr

Englisch Name:BCIP/NBT

CBNumberCB5448175

SummenformelC48H34BrClN11O10P

Molgewicht1071.18

MOL-DateiMol file

BCIP/NBT physikalisch-chemischer Eigenschaften

Flammpunkt	67 °C
storage temp.	-20°C
Aggregatzustand	tablet
Farbe	dark purple
Wasserlöslichkeit	water: 10mL, clear, yellow

Sicherheit

Kennzeichnung gefährlicher	Xi
R-Sätze:	36/37/38
S-Sätze:	26
WGK Germany	2
Speicherklasse	12 - Non Combustible Liquids
Hazard Classifications	Skin Sens. 1

Gefahreninformationscode (GHS)

BCIP/NBT Chemische Eigenschaften,Einsatz,Produktion Methoden

R-Sätze Betriebsanweisung:
R36/37/38:Reizt die Augen, die Atmungsorgane und die Haut.
S-Sätze Betriebsanweisung:
S26:Bei Berührung mit den Augen sofort gründlich mit Wasser abspülen und Arzt konsultieren.
Verwenden
A ready-to-use, precipitating substrate system for alkaline phosphatase. This substrate system produces an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually. Immunohistochemical staining with BCIP/NBT utilizes requires organic mounting media and can be counterstained with Nuclear Fast Red or Light Green. BCIP/NBT is generally a more sensitive method than Fast Red. Not recommended for ELISA (microwell) procedures.
Verwenden
SIGMAFAST BCIP/NBT has been used as a substrate
- for the anti-mouse IgG conjugated alkaline phosphatase in immunoblotting
- for alkaline phosphatase in the histochemical staining of primary osteoblasts
- for alkaline phosphatase staining in bone marrow stromal cells
Allgemeine Beschreibung
BM purple is a chromogenic substrate for alkaline phosphatase (AP) designed for precipitating enzyme immunoassays. It develops a permanent, dark purple band or spot at the AP binding site on the membrane or solid support. The stabilized substrate solution may be used directly from the bottle with no mixing or reconstitution. It gives less background staining than when using the BCIP/NBT two-bottle system, without a loss of sensitivity. The reaction product has a dark purple color and is insoluble in water. The dark purple color can be visually seen.

Appearance prior to use: light yellow color;
After color development: dark purple

BCIP/NBT Anbieter Lieferant Produzent Hersteller Vertrieb Händler.

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