Collagenase

Bezeichnung:Collagenase

CAS-Nr9001-12-1

Englisch Name:Collagenase

CBNumberCB2112176

SummenformelC60H100N2

Molgewicht849.4494

MOL-Datei9001-12-1.mol

Synonyma

Collagenase

Collagenase physikalisch-chemischer Eigenschaften

storage temp.	-20°C
Löslichkeit	Soluble in aqueous buffers.
Aggregatzustand	powder
Farbe	light brown
PH	7.0
Merck	2481
CAS Datenbank	9001-12-1
EPA chemische Informationen	Collagenase (9001-12-1)

Sicherheit

Kennzeichnung gefährlicher	Xn
R-Sätze:	36/37/38-42
S-Sätze:	22-24-26-36/37
WGK Germany	1
F	10-21
HS Code	3507907000

Gefahreninformationscode (GHS)

Collagenase Chemische Eigenschaften,Einsatz,Produktion Methoden

R-Sätze Betriebsanweisung:
R36/37/38:Reizt die Augen, die Atmungsorgane und die Haut.
R42:Sensibilisierung durch Einatmen möglich.
S-Sätze Betriebsanweisung:
S22:Staub nicht einatmen.
S24:Berührung mit der Haut vermeiden.
S26:Bei Berührung mit den Augen sofort gründlich mit Wasser abspülen und Arzt konsultieren.
S36/37:Bei der Arbeit geeignete Schutzhandschuhe und Schutzkleidung tragen.
Verwenden
This collagenase has been tested with cell lines to verify the product is not cytotoxic. Collagenase is typically used to digest the connective components in tissue samples to liberate individual cells. The concentration for cartilage dispersal is 1-2 mg/ml, but literature searches should be performed for species specific and/or tissue specific concentrations.
Verwenden
In investigation of the structure and biosynthesis of collagen; in dispersion of cells for tissue culture studies.
Verwenden
Collagenase, Type I, Clostridium histolyticum is used as a powder and solvent injection kit for the treatment of dupuytren's contracture.
Definition
A rare proteinase that catalyzes the hydrolysis of collagen. Collagenase activity has been demonstrated in several venoms of crotalid and viperid snakes and it occurs in certain species of clostridium.
Allgemeine Beschreibung
Collagenase from Clostridium histolyticum comprises of collagenases G and H. Collagenase has N-terminal activator domain and C-terminal peptidase domain. The collagen binding domains (CBD) and polycystic kidney disease-like (PKD-like) domain resides in the peptidase domain. The collagenase module undergoes structural changes upon calcium binding.
Biochem/physiol Actions
Effective release of cells from tissue requires the action of collagenase enzymes and the neutral protease. Collagenase is activated by four gram atom calcium (Ca2+) per mole enzyme. The culture filtrate is thought to contain at least 7 different proteases ranging in molecular weight from 68-130 kDa. The pH optimum is 6.3-8.8. The enzyme is typically used to digest the connective components in tissue samples to liberate individual cells. Ethylene glycol-bis(β-aminoethyl ether)-N,N,N′,N′-tetraacetic acid (EGTA)4; β-mercaptoethanol; glutathione, reduced; thioglycolic acid, sodium; and 2,2′-dipyridyl; 8-hydroxyquinoline are known to inhibit the enzyme activity.
läuterung methode
Collagenase is purified by using N-ethylmaleimide to activate the enzyme, and wheat germ agglutinin-agarose affinity chromatography [Callaway et al. Biochemistry 25 4757 1986].

Collagenase Anbieter Lieferant Produzent Hersteller Vertrieb Händler.

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