GLUTATHIONE S-TRANSFERASE

GLUTATHIONE S-TRANSFERASE Chemische Eigenschaften,Einsatz,Produktion Methoden

• Verwenden

  Glutathione S-Transferase from human placenta has been used in antigen microarray and informatics analysis.

• Allgemeine Beschreibung

  Glutathione S-transferase (GST) is a major detoxification enzyme, and exists as multiple cytoplasmic and membrane-bound isozymes. These isozymes differ in their catalytic activity, as well as in their non-catalytic binding properties. Cytoplasmic isoforms of GST are encoded by five genes, namely α, θ, μ, σ and π. α, μ and π are the most abundant forms in mammals. Membrane bound GST forms are encoded by a single gene.

• Biochem/physiol Actions

  Glutathione S-transferase (GST) catalyzes conjugation reactions during phase II metabolism. This enzyme also confers protection against catabolic products of peroxidized lipid and oxidized DNA, and ROS (reactive oxygen species) or electrophiles.

• läuterung methode

  It is purified by affinity chromatography using a column prepared by coupling glutathione to epoxy-saturated Sepharose. After washing contaminating proteins, the pure transferase is eluted with buffer containing reduced glutathione. The solution is then concentrated by ultrafiltration, dialysed against phosphate buffer at pH ~7 and stored in the presence of dithiothreitol (2mM) in aliquots at <-20o. [Simons & Vander Jag Anal Biochem 52 334 1977.]

GLUTATHIONE S-TRANSFERASE Anbieter Lieferant Produzent Hersteller Vertrieb Händler.

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