5-溴-2-（4-氟苯基）-3-（4-甲基磺酰基苯基）噻吩

生物活性靶点体外研究体内研究 5-溴-2-（4-氟苯基）-3-（4-甲基磺酰基苯基）噻吩试剂级价格

CAS号:88149-94-4
英文名:DUP-697
中文名:5-溴-2-（4-氟苯基）-3-（4-甲基磺酰基苯基）噻吩
CBNumber:CB8397180
分子式:C17H12BrFO2S2
分子量:411.31
MOL File:88149-94-4.mol

5-溴-2-（4-氟苯基）-3-（4-甲基磺酰基苯基）噻吩化学性质

储存条件 :2-8°C
溶解度 :DMF: 54 mg/ml; DMF:PBS (pH 7.2) (1:1): 0.5 mg/ml; DMSO: 15 mg/ml; Ethanol: 7 mg/ml
形态 :White to off-white solid.
颜色 :White to off-white

安全信息

危险品标志 :Xi
危险类别码 :36/37/38
安全说明 :26-36

5-溴-2-（4-氟苯基）-3-（4-甲基磺酰基苯基）噻吩性质、用途与生产工艺

生物活性 DuP-697 是邻位二芳基杂环的成员，并且是一种有效，不可逆，选择性和口服活性的 COX-2 抑制剂 (对于人 COX-2 和 COX-1，IC50 分别为 10 nM 和 800 nM)。DuP-697 对 HT29 大肠癌细胞具有抗增殖作用 (IC50 为 42.8 nM)，抗血管生成和促凋亡作用。DuP-697 可抑制前列腺素的合成，并具有抗炎，抗癌和退烧的作用。
靶点

hCOX-2

10 nM (IC ₅₀ )

hCOX-1

800 nM (IC ₅₀ )

体外研究

DuP-697 (0-100 nM; 24 hours; HT29 cells) treatment shows antiproliferative with an IC ₅₀ value of 42.8 nM.
DuP-697 (25-100 nM; 72 hours; HT29 cells) treatment causes concentration dependent apoptosis in HT29 cells. The percentage of UR (apoptosis portion) area increases gradually according to the concentration of DuP-697 from 7% in control group to 52% in 100 nM DuP-697.
DuP-697 in 100, 10 and 1 nM concentrations cause antiangiogenic effect. Antiangiogenic scores of DuP-697 are 1.2, 0.8 and 0.5, respectively.

Cell Proliferation Assay

Cell Line:	HT29 cells
Concentration:	0 nM, 12.5 nM, 25 nM, 50 nM, 100 nM
Incubation Time:	24 hours
Result:	Exhibited decreasing CI values in a concentration dependent manner. Showed statistically significant cytotoxic effect.

Apoptosis Analysis

Cell Line:	HT29 cells
Concentration:	25 nM, 50 nM, 100 nM
Incubation Time:	72 hours
Result:	Caused concentration dependent apoptosis in HT29 cells.

体内研究

DuP-697 is a potent inhibitor of paw swelling in nonestablished and established adjuvant arthritis in rats (ED ₅₀ = 0.03 and 0.18 mg/kg/day, respectively). DuP-697 has no effect on phenylquinone writhing in rats (ED ₅₀ greater than 100 mg/kg), but is analgetic against inflammation-related pain in the Randall-Selitto assay (ED ₅₀ = 3.5 mg/kg) and is a very potent antipyretic agent (ED ₅₀ = 0.05 mg/kg). DuP-697 (5 mg/kg i.v.) does not alter renal blood flow or the renal vascular response to angiotensin II in furosemide-pretreated, volume-depleted rats.
DuP-697 is a moderate inhibitor of bull seminal vesicle prostaglandin (PG) synthesis (IC ₅₀ of 24 μM) and a potent inhibitor of rat brain PG synthesis (IC ₅₀ of 4.5 μM) but was ineffective against rat kidney PG synthesis (IC ₅₀ of 75 μM).