L-硒代蛋氨酸

生物活性靶点体外研究体内研究 L-硒代蛋氨酸试剂级价格

L-硒代蛋氨酸

CAS号:3211-76-5
英文名:L-Selenomethionine
中文名:L-硒代蛋氨酸
CBNumber:CB8183929
分子式:C5H11NO2Se
分子量:196.11
MOL File:3211-76-5.mol

L-硒代蛋氨酸化学性质

熔点 :265 °C
比旋光度 :18 º (c=1, 1N HCl)
沸点 :320.8±37.0 °C(Predicted)
折射率 :18 ° (C=0.5, 2mol/L HCl)
储存条件 :-20°C
溶解度 :H₂O: 50 mg/mL
形态 :powder
酸度系数(pKa) :2.26±0.10(Predicted)
颜色 :white to off-white
水溶解性 :soluble
Merck :14,8441
InChI :InChI=1S/C5H11NO2Se/c1-9-3-2-4(6)5(7)8/h4H,2-3,6H2,1H3,(H,7,8)/t4-/m0/s1
InChIKey :RJFAYQIBOAGBLC-BYPYZUCNSA-N
SMILES :C(O)(=O)[C@@H](N)CC[Se]C
LogP :0.152 (est)
CAS 数据库 :3211-76-5(CAS DataBase Reference)

安全信息

危险品标志 :T,N
危险类别码 :23/25-33-50/53
安全说明 :20/21-28-45-60-61-28A
危险品运输编号 :UN 3283 6.1/PG 2
WGK Germany :3
RTECS号 :EK7713840
F :10
危险等级 :6.1
包装类别 :III
海关编码 :29309090

L-硒代蛋氨酸 MSDS

L-(+)-Selenomethionine

L-硒代蛋氨酸性质、用途与生产工艺

生物活性 L-SelenoMethionine 是 Selenomethionine 的 L-异构体，是硒的主要天然食物形式。L-SelenoMethionine 是一种癌症化学预防剂，可以通过饮食补充降低癌症的发生率并诱导癌细胞凋亡。L-SelenoMethionine 还可以增加谷胱甘肽过氧化物酶的表达。
靶点

Human Endogenous Metabolite

体外研究

L-SelenoMethionine (1-500 μM; 24-72 h) is selectively inhibits the growth of prostate cancer cells, compared with normal cells.
L-SelenoMethionine (500 μM; 48 h) induce apoptosis in prostate cancer cells.
L-SelenoMethionine (500 μM; 48 h) causes an increase in arrest in the G2-M phase of the cell cycle selectively in prostate cancer cells.
L-SelenoMethionine (5 μM; 24 h) mitigates gene expression associated with the cellular stress response from 10 cGy irradiation.

Cell Viability Assay

Cell Line:	Prostate cancer cells (LNCaP, PC-3, and DU145) and normal prostate cells (PrEC, PrSM, and PrSt)
Concentration:	1, 5, 10, 50, 100, 500 μM
Incubation Time:	24, 48, 72 hours
Result:	Inhibited the growth of prostate cancer cells, with a lower IC ₅₀ (1-90 μM) at 72 h than normal prostate cells (>500 μM).

Apoptosis Analysis

Cell Line:	Prostate cancer cells (LNCaP, PC-3, and DU145) and normal prostate cells (PrEC, PrSM, and PrSt)
Concentration:	500 μM
Incubation Time:	48 hours
Result:	Exhibited the highest level of DNA condensation in androgen-responsive LNCaP carcinoma cells, followed by PC-3 and DU145 cells. Exhibited the nicked-end DNA labeling in prostate cancer cells. Promoted the PARP cleavage in prostate cancer cells.

Cell Cycle Analysis

Cell Line:	Prostate cancer cells (LNCaP, PC-3, and DU145) and normal prostate cells (PrEC, PrSM, and PrSt)
Concentration:	500 μM
Incubation Time:	48 hours
Result:	Increased the sub-G0-G1 cell fraction of LNCaP (41.5%), PC-3 (12.1%), and DU145 cells (11.2%). Caused a significant increases in G2 cells , particularly in LNCaP (13%), PC-3 (32%), and DU145 (20%) cells.

RT-PCR

Cell Line:	Human thyroid epithelial cells (HTori-3)
Concentration:	5 μM
Incubation Time:	24 hours
Result:	Downregulated the CDC6, GADD45A, FAS and ATF3 in irradiated cells.

体内研究

L-SelenoMethionine (0.06-12 μg/g diet; p.o. for 3 d) partially or completely prevent the decrease in the serum or plasma levels of total antioxidants in rats exposed to gamma rays, protons or HZE particles.
用途可以用于蛋白质的结构分析。拿此产品来饲养细菌，这样最终形成的蛋白质里的特定位置就会被硒所取代，便于结构测定