抑二肽素A

生物活性靶点体外研究体内研究抑二肽素A 试剂级价格

抑二肽素A

CAS号:90614-48-5
英文名:DIPROTIN A
中文名:抑二肽素A
CBNumber:CB6261540
分子式:C17H31N3O4
分子量:341.45
MOL File:90614-48-5.mol

抑二肽素A化学性质

沸点 :583.1±50.0 °C(Predicted)
密度 :1.140±0.06 g/cm3(Predicted)
储存条件 :-20°C
溶解度 :DMSO:250.0(Max Conc. mg/mL);732.17(Max Conc. mM)
Water:67.08(Max Conc. mg/mL);196.44(Max Conc. mM)
形态 :White to off-white solid.
酸度系数(pKa) :3.72±0.22(Predicted)
水溶解性 :Soluble in water at 1mg/ml
CAS 数据库 :90614-48-5(CAS DataBase Reference)

安全信息

WGK Germany :3
RTECS号 :NR4737000

抑二肽素A性质、用途与生产工艺

生物活性 Diprotin A (Ile-Pro-Ile) 是二肽基肽酶 IV (DPP-IV) 的抑制剂。
靶点

IC50: DPP-IV

体外研究

Diprotin A (100 μM; 30 minutes after CXCR4-blocker or Src-inhibitor treatment) induces the phosphorylation of Src [Tyr 416] and VE-cadherin [Tyr731] in hECs in both normoxia and H/R conditions in human endothelial cells and disrupts endothelial cell-to-cell junctions, which are attenuated by CXCR4 (receptor of SDF-1α)-blocker or Src-inhibitor.

Western Blot Analysis

Cell Line:	Human endothelial cells ^[1]
Concentration:	100 μM
Incubation Time:	30 minutes after CXCR4-blocker or Src-inhibitor treatment
Result:	Induced the phosphorylation of Src [Tyr 416] and VE-cadherin [Tyr731] in hECs.

体内研究

Diprotin A (intraperitoneal injection; 70 μg/kg; twice daily; 7 days) increases the phosphorylation of Src and VE-cadherin and aggravates vascular leakage in the retinas. Collectively, Diprotin A induces vascular leakage by augmenting the SDF-1α/CXCR4/Src/VE-cadherin signaling pathway.

Animal Model:	Streptozotocin-induced diabetic retinopathy model in wild-type C57/BL6 mice
Dosage:	70 μg/kg
Administration:	Intraperitoneal injection; twice daily; 7 days
Result:	Induced vascular leakage by augmenting the SDF-1α/CXCR4/Src/VE-cadherin signaling pathway.