地榆皂苷I

概述生物活性体外研究

地榆皂苷I

CAS号:35286-58-9
英文名:3-O-alpha-L-Arabinopyranosylpomolic acid beta-D-glucopyranosyl ester
中文名:地榆皂苷I
CBNumber:CB52462159
分子式:C41H66O13
分子量:766.95
MOL File:35286-58-9.mol

地榆皂苷I化学性质

熔点 :198-202 ºC
沸点 :841.9±65.0 °C(Predicted)
密度 :1.35
储存条件 :-20°C, protect from light
溶解度 :Soluble in acetonitrile and methanol;
形态 :powder
酸度系数(pKa) :12.52±0.70(Predicted)
颜色 :White
水溶解性 :insoluble in water

地榆皂苷I性质、用途与生产工艺

概述地榆皂苷I，英文名ziyu-glycoside I，是从蔷薇科地榆属植物地榆或长叶地榆的根中提取得到的有明显药理活性的化合物。
生物活性 Ziyuglycoside I 从 S. officinalis 根中分离，具有抗皱活性，并增加 I 型胶原蛋白的表达。 Ziyuglycoside I 可用作化妆品的有效成分。Ziyuglycoside I 引发 p53 介导的细胞周期阻滞和凋亡 (apoptosis)，可用于三阴乳腺癌的相关研究。

体外研究

Ziyuglycoside I (5-160 µM; 24 hours) reveals a marked anti-proliferation activity with an IC ₅₀ value of 13.96 µM in MDA-MB-231 cells. Ziyuglycoside I (5-20 µM; 24 hours) induces MDA-MB-231 cell apoptosis by increasing the percentage of apoptotic cells from 2.43% to 44.76% at 20 µM. Ziyuglycoside I (5-20 µM; 24 hours) induces the cleavage of caspas-8, caspase-9, and caspase-3, up-regulates the pro-apoptotic protein Bax, and down-regulates anti-apoptotic protein Bal-2, dose-dependently reduces the level of mito-cyto c expression.

Cell Viability Assay

Cell Line:	MDA-MB-231 cells
Concentration:	5 µM, 10 µM, 20 µM, 40 µM, 80 µM, and 160 µM
Incubation Time:	24 hours
Result:	Showed cytotoxicity on MDA-MB-231 cells.

Cell Cycle Analysis

Cell Line:	MDA-MB-231 cells
Concentration:	5 µM, 10 µM, and 20 µM
Incubation Time:	24 hours
Result:	Induced G2/M phase arrest and apoptosis on MDA-MB-231 Cells

Western Blot Analysis

Cell Line:	MDA-MB-231 cells
Concentration:	5 µM, 10 µM, and 20 µM
Incubation Time:	24 hours
Result:	Induced apoptosis in MDA-MB-231 Cells through Intrinsic and Extrinsic Pathways.