3,4-二羟基苄胺·氢溴酸

生物活性体外研究体内研究 3,4-二羟基苄胺·氢溴酸试剂级价格

3,4-二羟基苄胺·氢溴酸

CAS号:16290-26-9
英文名:3,4-DIHYDROXYBENZYLAMINE HYDROBROMIDE
中文名:3,4-二羟基苄胺·氢溴酸
CBNumber:CB2436975
分子式:C7H10BrNO2
分子量:220.06
MOL File:16290-26-9.mol

3,4-二羟基苄胺·氢溴酸化学性质

熔点 :184-186 °C(lit.)
储存条件 :Inert atmosphere,Room Temperature
溶解度 :DMSO (Slightly), Methanol (Very Slightly)
形态 :Crystalline Powder
颜色 :Light beige
敏感性 :Hygroscopic
BRN :4002646
CAS 数据库 :16290-26-9(CAS DataBase Reference)

安全信息

危险品标志 :Xi
危险类别码 :36/37/38
安全说明 :26-36
WGK Germany :3
F :10
海关编码 :29222900

3,4-二羟基苄胺·氢溴酸性质、用途与生产工艺

生物活性 3,4-Dihydroxybenzylamine hydrobromide (NSC 263475 hydrobromide) 是一种结构改良的多巴胺类似物细胞毒物，抑制黑色素瘤细胞中 DNA 聚合酶 (DNA polymerase) 活性。3,4-Dihydroxybenzylamine hydrobromide 在不同程度的酪氨酸酶活性的黑色素瘤细胞系中显示不同的生长抑制活性。

体外研究

3,4-Dihydroxybenzylamine hydrobromide (1 μM-10 mM; 48 hours) inhibits the growth of all melanoma cell lines with an IC ₅₀ of 100 μM, while the SK-MELB cell line exhibits an IC ₅₀ of 122 μM. The IC ₅₀ values are 30, 84,90, and 68 μM for Human SK-MEL-30, SK-MEL-2, SK-MEL-3,RPMI-7951 cells, respectively. It exhibits IC ₅₀ values of 10, 25, 67, and 184 μM for S91A,S91B, L1210, and SCC-25 cells, respectively.3,4-Dihydroxybenzylamine hydrobromide (1 μM-10 mM; 48 hours) cytotoxicity in SK-MEL-2 and SK-MEL-28 cell lines can be enhanced by application of buthionine sulfoximine (BSO). BSO treatment sensitizes marginally pigmented cells to the effects of 3,4-DHBA, and the presence of decreses IC ₅₀ values of 3,4-DHBA by 127% in the SK-MEL-28 cell line and by 148% in the SK-MEL-2 cell line.

Cell Viability Assay

Cell Line:	Human and Murine Melanoma Cell Lines; Non-Melanoma Cell Lines
Concentration:	1 μM-10 mM
Incubation Time:	48 hours
Result:	Inhibited melanoma cell Lines growth.

体内研究

3,4-Dihydroxybenzylamine hydrobromide (intraperitoneal injection; 1000 mg/kg; 7 days) has the least toxic effect in non-tumor-bearing B6D2F1 mice,and mice is tolerated at this dose. 3,4-Dihydroxybenzylamine hydrobromide (intraperitoneal injection; 200-800 mg/kg; 21 days) has different effects at different doses, the median life-span are 17, 24.5, 26, 29 and 25 days for 0 mg/kg, 200 mg/kg, 400 mg/kg, 600 mg/kg, and 800 mg/kg, respectively.

Animal Model:	C57BL/6 mice with B6D2F1 cells
Dosage:	200 mg/kg, 400 mg/kg, 600 mg/kg, and 800 mg/kg
Administration:	Intraperitoneal injection
Result:	Exhibited increased life-span of 44%,46%, 70% and 50% for 200 mg/kg, 400 mg/kg, 600 mg/kg, and 800 mg/kg, respectively.