92-32-0
基本信息
吡罗红Y
哌咯寧Y
派若宁Y
派洛宁Y
派洛宁G
吡罗红 G
吡啰红Y(G)
二苯氧杂已胺G
派洛宁Y/吡罗红G
pyronine
PYRONIN G
PYRONIN J
PYRONIN Y
PYRONIN GS
PYRONINE Y
PYRONINE G
pyroninezh
CI NO 45005
物理化学性质
| 熔点 | 250-260 °C(lit.) |
| 熔点 | 250-260 °C(lit.) |
| 储存条件 | room temp |
| 储存条件 | Store at RT. |
| 溶解度 | 易溶于水；微溶于乙醇、乙二醇、甲基溶纤剂 |
| 色指数 | 45005 |
| 形态 | 粉末/固体 |
| 颜色 | 粉色 |
| 水溶解性 | Soluble in water. Also soluble in methyl cellosolve (9 mg/mL) and ethanol (3 mg/mL). |
| 最大波长(λmax) | 548 nm |
| Merck | 13,8097 |
| BRN | 3795789 |
| 稳定性 | 稳定的。与强氧化剂不相容。 |
| 生物领域应用 | Antimalarial agent; antiviral agent; nucleic acid sequencing; treating protozoan infections; herbicides |
| InChIKey | INCIMLINXXICKS-UHFFFAOYSA-M |
| CAS 数据库 | 92-32-0 |
| EPA化学物质信息 | Pyronine Y (92-32-0) |
安全数据
| 危险性符号(GHS) |  GHS08 |
| 警示词 | 危险 |
| 危险性描述 | H341b |
| 防范说明 | P201-P202-P281-P308+P313-P403-P501c |
| 危险品标志 | Xn,Xi |
| 危险类别码 | 68-36/37/38 |
| 安全说明 | 22-24/25-36-26 |
| 安全说明 | 22-24/25-36-26 |
| WGK Germany | 3 |
| WGK Germany | 3 |
| RTECS号 | BQ1450000 |
| TSCA | Yes |
| 海关编码 | 32041300 |
应用领域
吡啰红Y价格(试剂级)
| 报价日期 | 产品编号 | 产品名称 | CAS号 | 包装 | 价格 |
| 2024/11/08 | 41863 | 派洛宁Y Pyronin Y, pure, certified | 92-32-0 | 1g | 410元 |
| 2024/11/08 | 20010 | 派洛宁Y Pyronin Y, pure, high purity biological stain | 92-32-0 | 1g | 595元 |
| 2024/11/08 | 41863 | 派洛宁Y Pyronin Y, pure, certified | 92-32-0 | 5g | 1530元 |
常见问题列表
Pyronin Y forms fluorescent complexes with double-stranded nucleic acids, especially RNA, enabling semi-quantitative analysis of cellular RNA in flow cytometry, to estimate the RNA content per cell in formalin fixed EL4 leukosis tumor cells, enzyme dispersed R3327-G rat prostatic adenocarcinoma cells, mouse spleen cells stimulated with concanavalin A, and human peripheral blood lymphocytes stimulated with phytohemagglutinin.
A fluorescent staining procedure based on pyronin Y is described. The technique has been used to stain RNA in human reticulocytes for subsequent flow analysis and sorting.
In viable cells this dye also accumulates in mitochondria. At a concentration of 1.7 to 3.3 μM, pyronin Y is localized almost exclusively in mitochondria of cultured cells, similar to another mitochondria! probe, rhodamine 123. At that concentration PY is not toxic but suppressed cell growth, arresting cells.
Pyronin Y has long been used, in combination with other dyes such as Methyl Green, as a differential stain for nucleic acids in paraffin tissue sections. In sections stained with Methyl Green-pyronin Y, red blood cells, elastic fibre of blood vessels, zymogen granules of pancreatic acinar cells, surface membrane of heptocytes and kidney tubular cells showed strikingly strong green and/or red fluorescence, while the nuclei of cells appeared non-fluorescent.
Pyronin Y binds to double stranded RNA (dsRNA) including messenger RNA (mRNA), transfer RNA (tRNA) and ribosomal RNA (rRNA).