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494753-69-4

供应商 相关产品 基本信息 理化性质 常见问题列表 应用领域

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基本信息

英文
Ginsenoside RK2
中文
人参皂苷RK2
英文名称
PAN 30
Ginsenoside RK2 USP/EP/BP
Ginsenoside Rk1 (Rk1:Rz1=5:2)
3beta,12beta-Dihydroxydammar-20(21),24-diene-3-O-beta-D-glucopyranosyl(1→
3β,12β-dihydroxydammar-20(21),24-diene-3-O-β-D-glucopyranosyl(1->2)-β-D-glucopyranoside
β-D-Glucopyranoside, (3β,12β)-12-hydroxydammara-20,24-dien-3-yl 2-O-β-D-glucopyranosyl-
3beta,12beta-Dihydroxydammar-20(21),24-diene-3-O-beta-D-glucopyranosyl(1-2)-beta-D-glucopyranoside
CAS
494753-69-4
中文名称
人参皂苷RK1(RK1:RZ1=5:2)
分子式
C42H70O12
分子量
767
MOL 文件
494753-69-4.mol

理化性质

外观性状
白色粉末,可溶于甲醇、乙醇、DMSO等有机溶剂,来源于人参。
沸点 
854.5±65.0 °C(Predicted)
密度 
1.27±0.1 g/cm3(Predicted)
储存条件 
Sealed in dry,2-8°C
酸度系数(pKa)
12.85±0.70(Predicted)

常见问题列表

用途

Rk2一般通过水解人参皂苷二醇组获得,但水解成分复杂,不利于分离并准确鉴定结构。本文通过酶法水解高纯度的人参皂苷Rk2,水解产物通过高分辨质谱和核磁确证。


功效与作用
人参皂苷Rk2能有效抑制肿瘤细胞生长,诱导分化癌细胞,具有抗浸润和抗转移疗效。
生物活性
Ginsenoside Rk1 人参皂苷 Rk1 是人参的高温加工提取物。 Ginsenoside Rk1 具有抗炎作用,抑制 Jak2/Stat3 信号通路和 NF-κB 的激活。 Ginsenoside Rk1 具有抗肿瘤作用,抗血小板聚集活性,抗炎作用,抗胰岛素抵抗,肾保护作用,抗菌作用,认知功能增强,脂质积聚减少和预防骨质疏松症。 Ginsenoside Rk1 通过触发细胞内活性氧 (ROS) 生成和阻断 PI3K/Akt 途径诱导细胞凋亡。
体外研究

Ginsenoside Rk1 (0-40 μM; 6 hours) inhibits MCP-1 and TNF-α mRNA induced by lipopolysaccharide (LPS), expression of IL-1β is inhibited at 40 μM. Ginsenoside Rk1 (0-40 μM; 24 hours) inhibits phosphorylation of JAK2 and STAT3 (Tyr705 and Ser727) in LPS-induced RAW264.7 cells in a dose-dependent manner. Ginsenoside Rk1 (0-160 μM; 48 hours) results in cell viability significant decrease 75.52 ± 2.51% (40 μM), 52.72 ± 2.54% (80 μM), 17.41 ± 2.94% (120 μM)and 12.63 ± 3.24% (160 μM) compared with control. Ginsenoside Rk1 (0-120 μM; 24 hours) increases G0/G1 phase proportion accompanied with S and G2/M phase proportion decrease in MDA-MB-231 cells. Ginsenoside Rk1 (0-120 μM; 24 hours) promotes the percentage of apoptotic cells in a dose-dependent manner, exhibits to reduction of cell number with nucleus fragmentation, condensation and apoptotic body formation.

RT-PCR

Cell Line: RAW264.7 cells
Concentration: 10 μM, 20 μM, 40 μM
Incubation Time: 6 hours
Result: Inhibited JAK2-dependent STAT3 activation in LPS-activated RAW264.7 cells.

Western Blot Analysis

Cell Line: RAW264.7 cells
Concentration: 10 μM, 20 μM, 40 μM
Incubation Time: 6 hours
Result: Inhibited JAK2-dependent STAT3 activation in LPS-activated RAW264.7 cells

Cell Viability Assay

Cell Line: MDA-MB-231 cells
Concentration: 0 μM, 40 μM, 80 μM, 120 μM
Incubation Time: 48 hours
Result: Inhibited MDA-MB-231 cells proliferation in a dose- and time-dependent manner.

Cell Cycle Analysis

Cell Line: MDA-MB-231 cells
Concentration: 0 μM, 40 μM, 80 μM, 120 μM
Incubation Time: 24 hours
Result: Induced G0/G1 phase arrest.

Apoptosis Analysis

Cell Line: MDA-MB-231 cells
Concentration: 0 μM, 40 μM, 80 μM, 120 μM
Incubation Time: 24 hours
Result: Induced apoptosis in MDA-MB-231 cells.

应用领域

用途1
用于含量测定/鉴定/药理实验等。
药理药效:抗癌、提高免疫功能、调节神经系统。
用途2
人参皂苷Rk1是蒸参中提取的成分之一,具有抗肿瘤的生物活性。
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