ChemicalBook CAS DataBase List 9007-81-2

9007-81-2

Name	FREUND'S COMPLETE ADJUVANT
CAS	9007-81-2
Molecular Formula	C9H12FN3O5
MDL Number	MFCD00131105

Chemical Properties

storage temp.	2-8°C
form	cell suspension
color	amber

Safety Data

Symbol(GHS)	GHS08
Signal word	Danger
Hazard statements	H304
Precautionary statements	P301+P310-P331-P405-P501
Safety Statements	23-24/25 less more
WGK Germany	3
RTECS	LS6860000

Hazard Information

Uses

Antigens (preferably in saline) are typically mixed with an equal volume of the adjuvant to form an emulsion.

Uses

Immunization

General Description

1 Set (10 x 10 ml) each containing yellow, oily, bacterial suspension.

Biochem/physiol Actions

The mycobacteria in Complete Freund′s adjuvant attracts macrophages and other cells to the injection site which enhances the immune response. For this reason, Complete Freund′s Adjuvant is used for initial injections and Incomplete Freund′s Adjuvant for subsequent boosts.

in vivo

Complete Freund's adjuvant (CFA) induces experimental autoimmune myocarditis and neuropathic pain, with a relatively low plasma clearance rate (22%)^[1]^[2]^[3]^[4].

Induction of experimental autoimmune myocarditis (EAM)^[1]

Background

Complete Freund's adjuvant (CFA) induces myocarditis by activating the immune system and enhancing the antigen-specific immune response.

Specific Modeling Methods

Mice: A/J, BALB/c, and IL6KO BALB/c ? male ? 6-8-week-old
Administration: 5mg/ml ? s.c. ? twice

Note

(1) On day 0, all mice additionally received 500 ng of pertussis toxin (i.p.)
(2) On days 0 and 7, mice received subcutaneous injections peptide emulsified in CFA supplemented with 5 mg/mL of Mycobacterium tuberculosis, strain H37Ra.

Modeling Indicators

Histological analysis: Myocarditis severity was assessed by histology and relative heart weight.
Molecular changes: Test serum levels of total IgG, IgG1, IgG2a, IgG2b, IL-1b and IL6.

Correlated Product(s): Incomplete Freund's adjuvant (IFA) (HY-153808A); Complete Freund's adjuvant (CFA, 1 mg/ml) (HY-153808B)

Opposite Product(s): /

Induction of Neuropathic Pain^[2]^[3]

Background

Complete Freund's adjuvant (CFA) releases inflammatory mediators TNF-α and IL-6 in body which can activate peripheral nerve endings and act on pain nerve endings, resulting in neuropathic pain^[2]^[3].

Specific Modeling Methods

Mice: C57BL/6J ? male ? 8-week-old
Administration: 20 μl ? s.c. ? once

Note

(1) Inject Complete Freund’s Adjuvant (CFA) without dilution into the plantar area of the hind paw.
(2) Tests PWT and PWL for both hind paws corresponding to before and 24, 48, 72 h after CFA injection.

Modeling Indicators

Behavior Observation: Detect paw withdrawal threshold (PWT) and paw withdrawal latency (PWL).

Correlated Product(s): Galantamine (HY-76299)

Opposite Product(s): Mecamylamine (HY-B1395A)

Animal Model:	A/J, BALB/c, and IL6KO BALB/c male mice^[1]
Dosage:
Administration:	Subcutaneous Injectionon (sc); injected at days 0 and 7. EAM severity was analyzed by histology on day 21 (Repeated administration of CFA to animals can cause multiple injection-site effects, the most often observed are on site granulomas and pain)
Result:	Increased the relative proportion of CD11b+F4/80?monocytes and CD11b+Gr1+ granulocytes. Expanded monocytes in the heart and in the spleen and those monocytes in the spleen make less IL-10 as well as more IL-6.

Supplier

3B Pharmachem (Wuhan) International Co.,Ltd.

Telephone 18930552037

Websitehttps://www.chemicalbook.com/ShowSupplierProductsList13285/0_EN.htm

Shandong Xiya Chemical Co., Ltd

Telephone13355009207 13355009207

Websitehttp://www.xiyashiji.com

Sichuan Kulinan Technology Co., Ltd

Telephone400-1166-196 18981987031

Websitehttp://www.hx-r.com/

Sinopharm Chemical Reagent Co,Ltd.

Telephone86-21-63210123

Websitehttp://www.reagent.com.cn

Hunan Hui Bai Shi Biotechnology Co., Ltd.

Telephone0731-85526065 13308475853

Websitehttp://www.hnhbsj.com/

Beijing HuaMeiHuLiBiological Chemical

Telephone010-56205725

Websitehttp://www.huabeibiochem.com/

Shanghai Ruji Biology Technology Co., Ltd.

Telephone+86-21-65211385-8001 36031160

Websitehttp://www.chinaruji.com/

Shanghai Macklin Biochemical Co.,Ltd.

Telephone15221275939 15221275939

Websitehttp://www.macklin.cn

Sigma-Aldrich

Telephone021-61415566 800-8193336

Websitehttps://www.sigmaaldrich.cn

Beijing NuoqiYa Biotechnology Co., Ltd.

Telephone4000-819-385 010-62329685 13718666987

Websitehttp://www.bjoka-vip.com

Shanghai YuanYe Biotechnology Co., Ltd.

Telephone 15026964105

Websitehttps://www.shyuanye.com

Shandong Xiya Chemical Co., Ltd.

Telephone4009903999 13395398332

Websitehttp://www.xiyashiji.com

Guangdong wengjiang Chemical Reagent Co., Ltd.

Telephone0751-2886750 13927877953

Websitehttp://www.wj-chem.com

Anhui kuer Bioengineering Co., Ltd

Telephone0551-65171243 13856024262

Websitehttp://www.kuerhuaxue.com

Guangzhou LES biological Technology Co.,Ltd.

Telephone 13672434928;13672434928

Websitehttp://www.les64.com

Beijing Minruida Technology Co., Ltd.

Telephone010-82387566

Websitehttp://www.mreda.com.cn/

Hefei Bomei Biotechnology Co., Ltd.

Telephone13739298932 13856598764

Websitehttp://www.bomeibio.com

Hefei TNJ Chemical Industry Co.,Ltd.

Telephone+86-0551-65418671 +86-189 4982 3763

Websitehttps://www.tnjchem.com

1of4

Related Products

Send Inquriy