Chemical Properties
Off-white or yellow powder, freely soluble in water, pI 5.1, optimum pH 4.5-5.0. Stability: After six months at 4°C, activity decreases by 10%; after twelve months, activity decreases by 20%. Addition of butanol can increase stability; turbidity does not affect activity. Inhibitors include D-glucuronic acid, D-galacturonic acid, and organic peroxides. The absorption coefficient of a 1% aqueous solution is 17.0 at 280 nm. It rapidly hydrolyzes steroidal glucuronides and other glucuronides, but not α- or β-glucosides. The enzymatic reaction is: bound β-D-glucuronide + H??O═D-glucuronic acid + alcohol.
Uses
In the determination of urinary steroids and of steroid conjugates in blood.
Uses
β-glucuronidase is used as a reporter gene in GUS assays to monitor gene expression.
Uses
β-Glucuronidase was used in the study of Cer-β-glucuronide synthesis and evaluation of β-glucuronidase(s) from Escherichia coli or intestinal segments to release the head group.
General Description
β-Glucuronidase from Escherichia coli is similar to human glucuronidase enzyme and corresponds to molecular weight close to 69-71 kDa and has an pH optimum of 6.5-7.5. It belongs to family-2 glycosyl hydrolase and has active site residues glutamic acid 394, tyrosine 468 and glutamic acid 504.
Biochem/physiol Actions
β-Glucuronidase (GUSB) is a lysosomal enzyme, that degrades glucuronate-containing glycosaminoglycans. β-glucuronidase, obtained from abalone has the ability to hydrolyze samples, that has high analyte concentrations. Absence of β-glucuronidase results in mucopolysaccharidosis type VII (MPSVII), which leads to lysosomal storage in the brain.
