ChemicalBook CAS DataBase List 9001-06-3

9001-06-3

Name	CHITINASE
CAS	9001-06-3
EINECS(EC#)	232-578-7
Molecular Formula	C17H16N8Zn
MDL Number	MFCD00130771
Molecular Weight	397.753
MOL File	9001-06-3.mol

Chemical Properties

storage temp.	−20°C
solubility	0.05 M phosphate buffer, pH 6.0: soluble 0.90-1.10mg/mL, faintly hazy to hazy (with particles)
form	lyophilized powder
color	White to off-white
Water Solubility	H2O: soluble 0.90-1.10mg/mL
Specific Activity	≥600units/g solid
EPA Substance Registry System	Chitinase (9001-06-3)

Safety Data

Symbol(GHS)	GHS08
Signal word	Danger
Hazard statements	H334
Precautionary statements	P261-P284-P501
Safety Statements	36/39 less more
WGK Germany	3
RTECS	FM6205000
F	10-21

Hazard Information

Uses

Agriculture fields: control pathogens.
Human health care: Asthma.
Pharma: preparation of chitooligosaccharides and N-acetyl D glucosamine,
Preparation of single-cell protein
Isolation of protoplasts from fungi and yeast
Control of pathogenic fungi
Treatment of chitinous waste, mosquito control and morphogenesis

General Description

Chitinase is an extracellular complex of enzymes that degrade chitin. Chitin is a cell wall component of Fungi and exoskeketal essentials of different organisms which reshape their own chitin or digest/dissolve the chitin of other organisms (insects, fungi, yeast, and algae, and in the internal structures of other vertebrates) . Chitinases have been detected in many microorganisms and in plants. In fungi, chitinases assist in morphogenesis, to break down the inherent chitin content of fungal cell walls. Plant chitinases help in resistance to fungal attack and counteracting fungal growth, by targeting those same fungal cell walls. In bacteria, bacterial chitinases assist in utilizing chitin as a carbon source and as an energy source.Streptomyces griseus produces multiple chitinases of different molecular masses after growth induction with chitin as the carbon source.

The enzymatic hydrolysis of chitin to N-acetyl-D-glucosamine involves two consecutive enzyme reactions:

The first reaction, chitodextrinase-chitinase, is a poly(β-(1→4)-[2-acetamido-2-deoxy-D-glucoside])- glycanohydrolase, which removes chitobiose units from chitin.
The second activity is N-acetyl-glucosaminidasechitobiase, which cleaves the disaccharide to its monomer subunits, N-acetyl-D-glucosamine.

Biochem/physiol Actions

Chitinase serves as a biopesticide against several fungi and insects. This hydrolytic enzyme is capable of cleaving the glycosidic bonds in chitin.

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