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N-[4-(Methylamino)benzoyl]-L-glutamic acid zinc salt is used as a key intermediate in the synthesis of anti-folic acid agents such as methotrexate, and it has also been studied as an active ingredient with anti-inflammatory effects.

N-[4-(Methylamino)benzoyl]-L-glutamic acid zinc salt is used as a herbicide for the purpose of controlling and eliminating unwanted plant growth. It is particularly effective in targeting broadleaf and grassy weeds in various crops, such as soybeans, corn, and wheat. The application reason is its ability to disrupt the nitrogen metabolism in plants, causing their death and thus preventing competition for resources with the desired crops.