5719-85-7

4-hydroxy Tolbutamide is a cytochrome P450 2C8 (CYP2C8) and CYP2C9 metabolite of tolbutamide, a first-generation potassium channel blocker. Tolbutamide 4-hydroxylation is an often used probe reaction in the pharmaceutical industry for the characterization of CYP2C8 and CYP2C9 involvement in the metabolism of clinical drugs.

White Solid

A metabolite of Tolbutamide. Formed by the cytochrome CYP2CIIC8 and IIC9 subfamily of P450 enzymes

ChEBI: A urea that consists of 1-butylurea having a 4-hydroxymethylbenzenesulfonyl group attached at the 3-position.

Store at -20°C

[1] J O MINERS  D J B. Use of tolbutamide as a substrate probe for human hepatic cytochrome P450 2C9.[J]. Methods in enzymology, 1996, 272: 139-145. DOI: 10.1016/s0076-6879(96)72017-7
[2] VAN NGUYEN  Fawzy E  Michael Espiritu. Development and validation of a sensitive and specific LC-MS/MS cocktail assay for CYP450 enzymes: Application to study the effect of catechin on rat hepatic CYP activity.[J]. Biomedical Chromatography, 2020, 34 3: e4789. DOI: 10.1002/bmc.4789