464-92-6

16830-15-2

464-92-6

1) 1 g of (2α,3β,4α)-3,4-dihydroxy-6-(hydroxymethyl)-5-((3,4,5-trihydroxy-6-methyltetrahydro-2H-pyran-2-yl)oxy)tetrahydro-2H-pyran-2-yl)oxy)methyl)-3,4,5-trihydroxytetrahydro-2H-pyran-2-yl 10,11-dihydroxy-9-(hydroxymethyl)-hexamethyl- Eicosanoid-4a-carboxylic acid ester (asiaticoside), 4.3×10^-2 mol sodium dodecyl sulfate and 0.02 mol NaOH were mixed, water was added to 1 L, and stirred until completely dissolved to obtain a mixed solution. The hydrolysis reaction was carried out at room temperature (25°C) and atmospheric pressure (1 atm) for 15 min to obtain the hydrolysate.2) The pH of the hydrolysate was adjusted to 5.0 with 1 M HCl solution and allowed to stand for 30 min, followed by filtration and collection of the precipitate.3) The precipitate was dissolved in 70% (v/v) aqueous ethanol at a ratio of 1 g:30 mL and filtered to remove insoluble matter. Crystallization was carried out by adding 16 times the volume of distilled water to the filtrate and allowed to stand overnight for complete crystallization. The crystallized product was collected by filtration and dried to constant weight in a vacuum drying oven at 40°C to obtain cumaric acid white crystals of 0.445 g. The product was analyzed by high-performance liquid chromatography (HPLC) with a purity of 95.8% and a yield of 93.08%.