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Ammonium succinate is a water-soluble, colourless crystal with an acid taste that is used as a chemical intermediate in medicine, lacquers, and to make perfume esters. It is also used in foods as a sequestrant, buffer, and neutralizing agent.

Diammonium succinate (Ammonium succinate) is a hydrophobic hydrocarbon compound that can be used to study its interaction with microbial cells, especially the mechanisms of degradation and bioavailability. Diammonium succinate is a biomaterial or organic compound that can be used as a biomaterial or organic compound related to life science research.

A method of manufacturing a highly pure ammonium succinate solution including the steps of (A) producing calcium succinate trihydrate by crystallization fermentation of a microorganism; (B) converting calcium succinate trihydrate to calcium succinate monohydrate by transition crystallization; (C) separating the calcium succinate monohydrate crystals; (D) substituting the calcium salt in the calcium succinate monohydrate with ammonium salt resulting in an ammonium succinate solution; and (E) removing the solid calcium carbonate from the ammonium succinate solution.

[1] Bergmeyer H U, et al. Biochemical reagents[M]//Methods of Enzymatic Analysis. Academic Press, 1965: 967-1037.