17833-43-1

4-Methylumbelliferyl-α-D-glucopyranoside is a fluorogenic substrate for α-glucosidase. 4-Methylumbelliferyl-α-D-glucopyranoside is cleaved by α-glucosidase to release the fluorescent moiety 4-methylumbelliferyl (4-MU). 4-MU fluorescence is pH-dependent with excitation maxima of 320 and 360 nm at low (1.97-6.72) and high pH (7.12-10.3), respectively, and an emission maximum ranging from 445 to 455 nm, increasing as pH decreases. 4-Methylumbelliferyl-α-D-glucopyranoside has been used to quantify α-glucosidase activity in infant blood spot samples as a biomarker of Fabry and Pompe diseases, lysosomal storage disorders characterized by a deficiency in the enzyme.

white to off-white crystalline powder

4-Methylumbelliferyl-α-D-glucopyranoside is a fluorogenic substrate in the assay of a-glucosidase,it has been used in the study of Pompe disease. 

ChEBI: 4-Methylumbelliferyl-alpha-D-glucopyranoside is a member of coumarins and a glycoside.

Recrystallise 4-methylumbellifer-7-yl--D-glucopyranoside from hot H2O or EtOH. [Courtin-Duchateau & Veyrière Carbohydr Research 65 23, 29 1978, Beilstein 18 IV 443.]

[1] PETRA OLIVOVA . Effect of sample collection on α-galactosidase A enzyme activity measurements in dried blood spots on filter paper[J]. Clinica Chimica Acta, 2009, 403 1: Pages 159-162. DOI: 10.1016/j.cca.2009.02.008
[2] STEPHAN C.A. WENS . Remarkably low fibroblast acid α-glucosidase activity in three adults with Pompe disease[J]. Molecular genetics and metabolism, 2012, 107 3: Pages 485-489. DOI: 10.1016/j.ymgme.2012.09.003