76204-02-9

Off-White to Yellow Solid

This compound has been used for fluorometric assay of neuraminidase as well as fluorescent staining of sialidases in PAGE.

Used as a substrate in a fluorometric assay of neuraminidase.

4-Methylumbelliferyl-N-acetyl-α-D-neuraminic acid is a fluorogenic substrate of neuraminidases. 4-Methylumbelliferyl-N-acetyl-α-D-neuraminic acid is cleaved by neuraminidases to release the fluorescence moiety 4-methylumbelliferyl (4-MU). 4-MU fluorescence is pH-dependent with excitation maxima of 320 and 360 nm at low (1.97-6.72) and high (7.12-10.3) pH, respectively, and an emission maximum ranging from 445 to 455 nm, increasing as pH decreases. 4-Methylumbelliferyl-N-acetyl-α-D-neuraminic acid has been used to quantify neuraminidase activity in mammalian cells and clinical isolates of influenza virus.

Substrate for fluorometric assay of neuraminidase. Used for fluorescent staining of sialidases in PAGE.

[1] E MONTI. Expression of a novel human sialidase encoded by the NEU2 gene.[J]. Glycobiology, 1999, 9 12: 1313-1321. DOI: 10.1093/glycob/9.12.1313
[2] NICHOLAS M STAMATOS. Differential expression of endogenous sialidases of human monocytes during cellular differentiation into macrophages.[J]. FEBS Journal, 2005, 272 10: 2545-2556. DOI: 10.1111/j.1742-4658.2005.04679.x
[3] ALAN S CROSS. NEU1 and NEU3 sialidase activity expressed in human lung microvascular endothelia: NEU1 restrains endothelial cell migration, whereas NEU3 does not.[J]. The Journal of Biological Chemistry, 2012: 15966-15980. DOI: 10.1074/jbc.m112.346817
[4] N T WETHERALL. Evaluation of neuraminidase enzyme assays using different substrates to measure susceptibility of influenza virus clinical isolates to neuraminidase inhibitors: report of the neuraminidase inhibitor susceptibility network.[J]. Journal of Clinical Microbiology, 2003, 41 2: 742-750. DOI: 10.1128/jcm.41.2.742-750.2003