ChemicalBook CAS DataBase List 115-40-2

115-40-2

Name	Bromocresol Purple
CAS	115-40-2
EINECS(EC#)	204-087-8
Molecular Formula	C21H16Br2O5S
MDL Number	MFCD00011681
Molecular Weight	540.22
MOL File	115-40-2.mol

Synonyms

3',3''-DIBROMO-O-CRESOLSULFONPHTHALEIN 5',5''-DIBROMO-2-CRESOLSULFONEPHTHALEIN 5',5''-DIBROMO-O-CRESOLSULFONEPHTHALEIN 5',5'-DIBROMO-O-CRESOLSULFONEPHTHALEIN 5',5''-DIBROMO-O-CRESOLSULFONPHTHALEIN 5,5'-DIBROMO-O-CRESOLSULFONPHTHALEIN A,A-BIS(5-BROMO-4-HYDROXY-M-TOLYL)-A-HYDROXY-O-TOLUENESULFONIC ACID G-SULTONE BCP BROMCRESOL PURPLE BROMCRESOL PURPLE SULTONE FORM BROMOCRESOL PURPLE BROMOCRESOL PURPLE ETHANOL(20) BROMOCRESOL PURPLE TS DIBROMO-O-CRESOLSULFONPHTHALEIN 4,4’-(3h-2,1-benzoxathiol-3-ylidene)bis(2-bromo-6-methylphenol)s,s-dioxide 4,4’-(3h-2,1-benzoxathiol-3-ylidene)bis(2-bromo-6-o-cresol)s,s-dioxide 4,4’-(3H-2,1-Benzoxathiol-3-ylidene)bis[2-bromo-b-methylphend]S,S-dioxide bromocresol Bromocresolred dibromo-o-cresolphthaleinsulfone

Chemical Properties

Appearance	Crystalline
Melting point	240 °C (dec.) (lit.)
bulk density	515kg/m3
density	1.6509 (estimate)
Fp	36 °C
storage temp.	Store at RT.
solubility	ethanol: soluble1mg/mL
Boiling point	590.2±50.0 °C(Predicted)
form	Solid
pka	6.21, 6.3, 6.4(at 25℃)
color	Purplish-brown
PH	pH : 5.2～6.8
PH Range	5.2(yellow)-6.8(purple)
Stability:	Stable. Incompatible with strong oxidizing agents.
Water Solubility	practically insoluble
ε(extinction coefficient)	≥22000 at 418-424nm in methanol
λmax	419nm, 433nm, 591nm
Merck	14,1387
BRN	359618
Major Application	Waveguides, fuel cells, sensors, sol-gel technology, display device, falsification-proof security paper, paints, detergents, identifying fresh and stale rice, detecting lactic acid bacteria, dental materials, vaginal infection test method
InChIKey	ABIUHPWEYMSGSR-UHFFFAOYSA-N
CAS DataBase Reference	115-40-2(CAS DataBase Reference)
EPA Substance Registry System	115-40-2(EPA Substance)

Safety Data

Symbol(GHS)	GHS07
Signal word	Warning
Hazard statements	H315-H319-H335
Precautionary statements	P261-P280a-P304+P340-P305+P351+P338-P405-P501a-P264-P280-P302+P352+P332+P313+P362+P364-P305+P351+P338+P337+P313
Hazard Codes	Xi,Xn
Risk Statements	R36/37/38:Irritating to eyes, respiratory system and skin . R10:Flammable. R20/21/22:Harmful by inhalation, in contact with skin and if swallowed . less more
Safety Statements	S26:In case of contact with eyes, rinse immediately with plenty of water and seek medical advice . S36:Wear suitable protective clothing . S37/39:Wear suitable gloves and eye/face protection . S16:Keep away from sources of ignition-No smoking . less more
RIDADR	UN 1993 3/PG 3
WGK Germany	3
TSCA	Yes
HS Code	29349990
Hazardous Substances Data	115-40-2(Hazardous Substances Data)

Raw materials And Preparation Products

Hazard Information

Chemical Properties

Crystalline

Uses

As indicator, pH 5.2 yellow; pH 6.8 purple.

Definition

ChEBI: A member of the class of 2,1-benzoxathioles that is 2,1-benzoxathiole 1,1-dioxide in which both of the hydrogens at position 3 have been substituted by 3-bromo-4-hydroxy-5-methylphenyl groups. A hydrophilic dye that is used as a pH indicator and to measure serum albumin concentrations.

General Description

Bromocresol purple is a pH indicator, which changes color from yellow (at low pH 5.2) to violet (above pH 6.8). It aids in determining the dead cell count for cells with plasma membrane damage. Bromocresol purple is widely used as a fluorescent stain in yeast cells. Cells with an intact plasma membrane are not stained, while damaged cells are seen as blue-grey ghosts.

Biochem/physiol Actions

Bromocresol purple is a pH indicator which changes color from yellow (at low pH 5.2) to violet (above pH 6.8). It is mainly used as a fluorescent stain in yeast cells. It helps in dead cell count for cells with plasma membrane damage. Cells with proper cell membrane are not stained, while damaged cells are seen as blue-grey ghosts.

Purification Methods

Dissolve the dye in aqueous 5% NaHCO3 solution and precipitate it from a hot solution by dropwise addition of aqueous HCl. Repeat this until the UV/VIS-extinction did not increase at 419nm. It can also be recrystallised from *benzene. It is an indicator: at pH 5.2 (yellow) and pH 6.8 (purple). [Beilstein 19/3 V 460.]

Material Safety Data Sheet(MSDS)

Spectrum Detail

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