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1239-45-8

Name Ethidium bromide
CAS 1239-45-8
EINECS(EC#) 214-984-6
Molecular Formula C21H20BrN3
MDL Number MFCD00011724
Molecular Weight 394.31
MOL File 1239-45-8.mol

Chemical Properties

Appearance Purple/maroon crystalline powder
Melting point  260-262 °C (dec.)(lit.)
density  1.3739 (rough estimate)
refractive index  1.6700 (estimate)
Fp  >100°C
storage temp.  2-8°C
solubility  H2O: 10 mg/mL, opaque, strongly red
form  powder
color  Red to dark purple
Odor Odorless solid
Stability: Stable. Incompatible with strong oxidizing agents.
Water Solubility  40 g/L (25 ºC)
λmax 518 nm, 210 nm, 285 nm, 316 nm, 343 nm, 480 nm, 525 nm
Merck  14,4731
BRN  3642536
Biological Applications Nucleic acid hybridization; detecting nucleic acids,cells,cancer cells,human cytomegalovirus,hydrogenase A (hydA) of Clostridia,influenza A virus,oligonucleotides,viable Plesiomonas shigelloides; apoptosis assay; nucleic acid quantification
Major Application Electroluminescent displays;photoresists
CAS DataBase Reference 1239-45-8(CAS DataBase Reference)
EPA Substance Registry System 1239-45-8(EPA Substance)

Safety Data

Hazard Codes  T,T+
Risk Statements 
R23:Toxic by inhalation.
R68:Possible risk of irreversible effects.
R36/37/38:Irritating to eyes, respiratory system and skin .
R26:Very Toxic by inhalation.
R21/22:Harmful in contact with skin and if swallowed .
R22:Harmful if swallowed.
Safety Statements 
S36/37:Wear suitable protective clothing and gloves .
S45:In case of accident or if you feel unwell, seek medical advice immediately (show label where possible) .
S36/37/39:Wear suitable protective clothing, gloves and eye/face protection .
S26:In case of contact with eyes, rinse immediately with plenty of water and seek medical advice .
S22:Do not breathe dust .
S28:After contact with skin, wash immediately with plenty of ... (to be specified by the manufacturer) .
RIDADR  UN 2811 6.1/PG 1
WGK Germany  3
RTECS  SF7950000
8-9
HazardClass  6.1
PackingGroup  I
HS Code  29339990
Safety Profile
Poison by intraperitoneal and subcutaneous routes. Human mutation data reported. When heated to decomposition it emits very toxic fumes of NOx and Br-. See also BROMIDES.
Hazardous Substances Data 1239-45-8(Hazardous Substances Data)
Toxicity
Intercalating dye widely used to stain DNA in gels and gradients. The DNA can be visualized readily by irradiation with ultraviolet light, as little as 0.5 μg of DNA being detectable by such methods. Ethidium bromide is also added to cesium chloride density gradients, since as an intercalating molecule it binds more readily to linear DNA than to closed collinear circles of DNA (such as plasmids). Binding of ethidium bromide reduces the density of DNA, thus covalent circles of DNA have higher densities at saturating concentrations of ethidium bromide, permitting the separation of plasmid DNA. Ethidium bromide is a putative carcinogen.

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