MitoLite™ reagents are fluorogenic probes that can be used to label mitochondria within live cells. They are hydrophobic and easily cross live cell membranes to accumulate in mitochondria likely via the mitochondrial membrane potential gradient. MitoLite™ reagents contain a cell-retaining group, which allows them to remain in the mitochondria long-term, and their fluorescence can be measured using fluorescence microscopy, microplate fluorometry, or flow cytometry. MitoLite™ Deep Red FX660 displays excitation/emission maxima of 640/659 nm, respectively, and can be used for live and fixed cell applications.
