LysoBrite™ reagents are fluorogenic probes that can be used to label lysosomes within live cells. They are hydrophobic and easily cross live cell membranes to accumulate in lysosomes likely via the lysosomal pH gradient. LysoBrite™ fluorescence is enhanced in the acidic environment within the lysosome, and this fluorescence can be measured using fluorescence microscopy, microplate fluorometry, or flow cytometry. LysoBrite™ Blue exhibits excitation/emission maxima of 353/442 nm, respectively. It has been used to monitor endosomal escape of doxorubicin theranostic nanoparticles in HeLa cells.
