Recombinant Bovine enterokinas

上海更新日期：2013-01-06

Shanghai Yaxin Biotechnology Co. Ltd.

非会员

联系人：Tang Jiayi

电话：021-51718529拨打

邮箱：sales@yaxinbio.com

产品详情:

中文名称：: Recombinant Bovine enterokinas

英文名称：: Recombinant Bovine enterokinas

CAS号：: 9014-74-8

Recombinant Bovine enterokinase high specificity, high purity

DESCRIPTIONS

YaxinBio Enterokinase is a highly purified recombinant Bovine enterokinase. The enzyme has been extensively purified and there are no any other contaminating proteases. Enterokinase is a specific protease that cleaves a lysine Carboxy-terminal preceded by four aspartic acids: Asp-Asp-Asp-Asp-Lys. Enterokinase will not cleave, however, if this lysine is followed by a proline. Enterokinase can remove fusion tags located in the N-terminal section of proteins. Enterokinase is useful for removing unwanted tags.

SALIENT ADVANTAGES

High purity

One single main band at 35kD by SDS-PAGE

high specificity

1) specific protease that cleaves after a lysine preceded by four aspartic acids: Asp-Asp-Asp-Asp-Lys

2) No any other proteases contaminated

SDS-PAGE analysis of 1 U EK effect on 50ul substrate after 0min, 10min, 20min, 30min, 40min, 50min and 60min, respectively.

PRODUCTS INFORMATION

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Source

E.Coli

M.W.

Theoretical MW: 25,850 Da
The apparent MW on SDS-PAGE: about 27,000 Da

Specific Activity

One unit is defined as the amount of enzyme needed to cleave 50 ug of fusion protein in 16 hours to 95% completion at 22°C in a buffer containing 25 mM Tris-HCl, pH 8.0.

Storage

Store at -20°C after delivery.

Formulation

YaxinBio enterokinase, formulated using YaxinBio 's proprietary technology, can be shipped at room temperature. It will remain stable at 37°C for one week without losing any activity.

RELATIVE PRODUCTION: Recombinant Porcine Trypsin,

Recombinant Bovine enterokinase