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  • Recombinant Carboxypeptidase B

Recombinant Carboxypeptidase B

Recombinant Carboxypeptidase B
9025-24-5
上海 更新日期:2016-06-18

Shanghai Yaxin Biotechnology Co. Ltd.

非会员
联系人:Tang Jiayi
电话:021-51718529拨打
邮箱:sales@yaxinbio.com

产品详情:

中文名称:
Recombinant Carboxypeptidase B
英文名称:
Recombinant Carboxypeptidase B
CAS号:
9025-24-5
DESCRIPTIONS
Carboxypeptidase B catalyzes hydrolysis of the basicamino acids lysine, arginine and histidine from the Cterminal of polypeptides. The molecular weight is 34,500 daltons, the pH optimum is 8.0, and pI is 6.0. Carboxypeptidase B is competitively inhibited by arginine and lysine. The enzyme is inhibited by metal chelating agents, e.g., EDTA. Recombinant Carboxypeptidase B (EC 3.4.17.2) is expressed in E.Coli and purified by high pressure liquid chromatography. There is no foreign enzyme activity such as carboxypeptidase A and chymotrypsin. No protease inhibitors such as PMSF contain in preparation.
 
SALIENT ADVANTAGES
 
Animal origin free

Recombinant carboxypeptidase B eliminates the risk of viruses, or other potential adventitious agents found in animal derived carboxypepitdase B. Which belong to the AOF level 3 products.

 
Stable
A sterile recombinant carboxypeptidase B lyophilized powder eliminates the contamination opportunity and decreases the chance of activity loss in the process of transport and storage. Convenient for transport and store.
 
High purity
 
1)Recombinant carboxypeptidase B provides increased specificity and eliminates contaminating activities found in lower purity enzymes
2)No other proteases contaminated such as chymotrypsin, carboxypeptidase A, and the content of recombinant trypsin less than 10ppm.
SALIENT FEATURES

Source
E. Coli
Purified by
HPLC
Physical form
White or white-like lyophilized powder
Additives
Tris salts, NaCl salts and carbohydrates.
Protein content
35% 50%
Special activity
>170 units/mg pro.
Purity
> 95%
Foreign activity
No chymotrypsin, carboxypeptidase A, and other protease contaminant.
The content of recombinant trypsin is less than 10ppm. 

 
 
 
 
 
 
 
 
 
 
 
 
3)One single main band at 35kD by SDS-PAGE.
PRODUCTS INFORMATION

Product
Cat. No.
Special activity
Packaging
Manufacturer
Recombinant Carboxypeptidase B
RCPB01170
170 u/mg pro
10mg, bulk
YaxinBio
RCPB01100
100 u/mg pro
1g, bulk
YaxinBio

 
UNIT DEFINITIONOne Unit of carboxypeptid
ase B activity hydrolyzes one micromole of
hippuryl-L-arginine per minute at 25℃, pH 7.65.
 
RELATIVE PRODUCTION: Recombinant Porcine Trypsin
关键字: Recombinant Carboxypeptidase B;Carboxypeptidase B;enzyme
Recombinant Carboxypeptidase B

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