重组牛肠激酶

Recombinant Bovine enterokinase high specificity, high purity DESCRIPTIONS YaxinBio Enterokinase is a highly purified recombinant Bovine enterokinase. The enzyme has been extensively purified and there are no any other contaminating proteases. Enterokinase is a specific protease that cleaves a lysine Carboxy-terminal preceded by four aspartic acids: Asp-Asp-Asp-Asp-Lys. Enterokinase will not cleave, however, if this lysine is followed by a proline. Enterokinase can remove fusion tags located in the N-terminal section of proteins. Enterokinase is useful for removing unwanted tags. SALIENT ADVANTAGES High purity One single main band at 35kD by SDS-PAGE high specificity 1) specific protease that cleaves after a lysine preceded by four aspartic acids: Asp-Asp-Asp-Asp-Lys 2) No any other proteases contaminated SDS-PAGE analysis of 1 U EK effect on 50ul substrate after 0min, 10min, 20min, 30min, 40min, 50min and 60min, respectively. PRODUCTS INFORMATION Source E.Coli M.W. Theoretical MW: 25,850 Da The apparent MW on SDS-PAGE: about 27,000 Da Specific Activity One unit is defined as the amount of enzyme needed to cleave 50 ug of fusion protein in 16 hours to 95% completion at 22°C in a buffer containing 25 mM Tris-HCl, pH 8.0. Storage Store at -20°C after delivery. Formulation YaxinBio enterokinase, formulated using YaxinBio 's proprietary technology, can be shipped at room temperature. It will remain stable at 37°C for one week without losing any activity. RELATIVE PRODUCTION: Recombinant Porcine Trypsin,