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  • pIRES2-DsRed-Express 载体

pIRES2-DsRed-Express 载体

pIRES2-DsRed-Express
1280 2ug 起订
上海 更新日期:2024-07-02

上海泽叶生物科技有限公司

VIP5年
联系人:宛双凤
电话:021-61998551拨打
手机:13122364865 拨打
邮箱:sale1@shzysw.net

产品详情:

中文名称:
pIRES2-DsRed-Express 载体
英文名称:
pIRES2-DsRed-Express
保存条件:
常温运输
纯度规格:
99%
产品类别:
质粒/载体

pIRES2-DsRed-Express 载体

质粒类型:
哺乳动物表达载体
启动子:
CMV
表达水平:
克隆方法:
多克隆位点,限制性内切酶
载体大小:
5264 bp (查看载体序列)
5' 测序引物及序列:
CMV-F: CGCAAATGGGCGGTAGGCGTG
3' 测序引物及序列:
IRES-R: CCTCACATTGCCAAAAGACG
载体抗性:
Kanamycin(卡那霉素)
筛选标记:
Neomycin (新霉素)
产品编号
产品名称
规格
价格
ZY2068
pIRES2-DsRed-Express
2ug
 
pIRES2-DsRed-Express质粒图谱

pIRES2-DsRed-Express contains the internal ribosome entry site (IRES; 1, 2) of the encephalomyocarditis virus (ECMV) between the MCS and a mutant of the Discosoma sp. red fluorescent protein DsRed-Express coding region (3–5). This permits both the gene of interest (cloned into the MCS) and the DsRed-Express gene to be translated from a single bicistronic mRNA. pIRES2-DsRed-Express is designed for the efficient selection by flow cytometry of transiently transfected mammalian cells expressing DsRed-Express and the protein of interest. This vector can also be used to express DsRed-Express alone or to obtain stably transfected cell lines without timeconsuming drug and clonal selection.

DsRed-Express is a human codon-optimized (4) DsRed variant of Discosoma sp. red fluorescent protein (6). Its coding sequence contains nine amino acid substitutions which improve solubility of the protein and reduce the time from transfection to detection of red fluorescence (exitation and emission maxima = 557 nm and 579 nm, respectively). In addition, these substitutions reduce the level of residual green emission (5). Although DsRed-Express most likely forms the same tetrameric structure as wild-type DsRed, DsRed-Express displays a reduced tendency to aggregate (3, 5).

The MCS in pIRES2-DsRed-Express is between the immediate early promoter of cytomegalovirus (P CMV IE ) and the IRES sequence. SV40 polyadenylation signals downstream of the DsRed-Express gene direct proper processing of the 3' end of the bicistronic mRNA. The vector backbone also contains an SV40 origin for replication in mammalian cells expressing the SV40 T antigen. A neomycin-resistance cassette (Neo r ), consisting of the SV40 early promoter, the neomycin/ kanamycin resistance gene of Tn5, and polyadenylation signals from the herpes simplex virus thymidine kinase (HSV TK) gene, allows stably transfected eukaryotic cells to be selected using G418. A bacterial promoter upstream of this cassette expresses kanamycin resistance in E. coli. The pIRES2-DsRed-Express backbone also provides a pUC origin of replication for propagation in E. coli and an f1 origin for single-stranded DNA production.

特别提示:本公司的所有产品仅可用于科研实验,严禁用于临床医疗及其他非科研用途!

pIRES2-DsRed-Express 载体/pIRES2-DsRed-Express 质粒

公司简介

上海泽叶生物科技有限公司是一家服务于生命科学领域的高新技术企业,主要为科研机构、高校、院所及企业产品开发研究提供所需要的科研类试剂、耗材、仪器、技术服务等。包括分子生物学、免疫学、微生物学、细胞学、材料科学,通用试剂、药物合成试剂、手性化合物、催化剂及配体、分析试剂、生物试剂,检测试剂等等

成立日期 (8年)
注册资本 100万元整
员工人数 1-10人
年营业额 ¥ 100万以内
经营模式 贸易,试剂,定制,服务
主营行业 生物化工,有机原料,化学试剂,医药原料,技术服务

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