Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human CCR5 transfected murine BaF3 cells is less than 2.0ng/ml, corresponding to a specific activity of >5.0×105IU/mg.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Lyophilized from a 0.2μm filtered concentrated solution in PBS.
Endotoxin
Less than 0.1EU/μg of rHuLAG-1/CCL4L1 as determined by LAL method.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions with a NaCl concentration no less than 300mM.
Category
Chemokines
Background
C-C motif chemokine 4-like, also known as MIP-1-beta, is encoded by one of several cytokine genes clustered on the q-arm of chromosome 17 and is similar to CCL4 which inhibits HIV entry by binding to the cellular receptor CCR5. The human CCL4L1 cDNA encodes a 92 amino acid (a.a.) precursor with a 23a.a. signal sequence and it shares greater than 98%a.a. sequence identity with CCL4 and CCL4L2. Recombinant Human CCL4L1 also shares 96%a.a. sequence identity with rhesus CCL4 and approximately 80~90%a.a. sequence identity with bovine, murine, rabbit, and rat CCL4.