Recombinant Human SDF-1γ/CXCL12γ

Name

Recombinant Human SDF-1γ/CXCL12γ (Recombinant Human Stromal-Cell Derived Factor-1γ/CXCL12γ; rHuSDF-1γ/CXCL12γ)；重组人基质细胞衍生因子1γ/CXCL12γ

Synonyms

Stromal cell-derived factor 1; SDF-1; hSDF-1; C-X-C motif chemokine 12; Intercrine reduced in hepatomas; IRH; hIRH; Pre-B cell growth-stimulating factor; PBSF; CXCL12

Purity

>96% by SDS-PAGE and HPLC analyses.

Biological Activity

Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using PHA and rHuIL-2 activated human peripheral blood T-lymphocytes is in a concentration range of 30-100ng/ml.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH7.4.

Endotoxin

Less than 0.1EU/μg of rHuSDF-1γ/CXCL12γ as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.

Category

Chemokines

Background

CXCL12 also known as SDF-1 is belonging to the CXC chemokine family. It is encoded by the CXCL12 gene. In recently study, Human CXCL12 is expressed as six isoforms that differ only in the C-terminal tail. And all SDF-1 isoforms undergo proteolytic processing of the first two N-terminal amino acids. Human SDF-1γ is synthesized as a 119 amino acid (a.a.) precursor that contains a 21a.a. signal sequence and a 98a.a. mature region and the SDF-1γ C-terminus binds heparin in secreted SDF-1γ, or targets the isoform to the nucleolus in the absence of a signal sequence. On the cell surface, the receptor for this chemokine is CXCR4 and syndecan4. CXCL12 is strongly chemotactic for T-lymphocytes, monocytes, but not neutrophils. CXCL12 is a very important factor in carcinogenesis and the neovascularisation linked to tumor progression.

Amino Acid Sequence

GKPVSLSYRC PCRFFESHVA RANVKHLKIL NTPNCALQIV ARLKNNNRQV CIDPKLKWIQ EYLEKALNKG RREEKVGKKE KIGKKKRQKK RKAAQKRKN

产品编号

产品名称

包装

KL-6546-2μg

Recombinant Human SDF-1γ/CXCL12γ

2μg

KL-6546-10μg

Recombinant Human SDF-1γ/CXCL12γ

10μg

KL-6546-100μg

Recombinant Human SDF-1γ/CXCL12γ

100μg

KL-6546-1mg

Recombinant Human SDF-1γ/CXCL12γ

1mg

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说明书

1份