产品编号 |
产品名称 |
产品包装 |
产品价格 |
P6546-1mg |
Recombinant Human SDF-1γ/CXCL12γ |
1mg |
15444.00元 |
Species
|
Gene ID
|
Accession
|
Source
|
Length
|
MW
|
Tag
|
Human
|
6387
|
P48061
|
E. coli
|
99aa
|
11.6kDa
|
-
|
About this Antibody
|
Name
|
Recombinant Human SDF-1γ/CXCL12γ (Recombinant Human Stromal-Cell Derived Factor-1γ/CXCL12γ; rHuSDF-1γ/CXCL12γ);重组人基质细胞衍生因子1γ/CXCL12γ
|
Synonyms
|
Stromal cell-derived factor 1; SDF-1; hSDF-1; C-X-C motif chemokine 12; Intercrine reduced in hepatomas; IRH; hIRH; Pre-B cell growth-stimulating factor; PBSF; CXCL12
|
Purity
|
>96% by SDS-PAGE and HPLC analyses.
|
Biological Activity
|
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using PHA and rHuIL-2 activated human peripheral blood T-lymphocytes is in a concentration range of 30-100ng/ml.
|
Physical Appearance
|
Sterile Filtered White lyophilized (freeze-dried) powder.
|
Formulation
|
Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH7.4.
|
Endotoxin
|
Less than 0.1EU/μg of rHuSDF-1γ/CXCL12γ as determined by LAL method.
|
Reconstitution
|
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
|
Category
|
Chemokines
|
Background
|
CXCL12 also known as SDF-1 is belonging to the CXC chemokine family. It is encoded by the CXCL12 gene. In recently study, Human CXCL12 is expressed as six isoforms that differ only in the C-terminal tail. And all SDF-1 isoforms undergo proteolytic processing of the first two N-terminal amino acids. Human SDF-1γ is synthesized as a 119 amino acid (a.a.) precursor that contains a 21a.a. signal sequence and a 98a.a. mature region and the SDF-1γ C-terminus binds heparin in secreted SDF-1γ, or targets the isoform to the nucleolus in the absence of a signal sequence. On the cell surface, the receptor for this chemokine is CXCR4 and syndecan4. CXCL12 is strongly chemotactic for T-lymphocytes, monocytes, but not neutrophils. CXCL12 is a very important factor in carcinogenesis and the neovascularisation linked to tumor progression.
|
Amino Acid Sequence
|
GKPVSLSYRC PCRFFESHVA RANVKHLKIL NTPNCALQIV ARLKNNNRQV CIDPKLKWIQ EYLEKALNKG RREEKVGKKE KIGKKKRQKK RKAAQKRKN
|
包装清单:
产品编号
|
产品名称
|
包装
|
P6546-2μg
|
Recombinant Human SDF-1γ/CXCL12γ
|
2μg
|
P6546-10μg
|
Recombinant Human SDF-1γ/CXCL12γ
|
10μg
|
P6546-100μg
|
Recombinant Human SDF-1γ/CXCL12γ
|
100μg
|
P6546-1mg
|
Recombinant Human SDF-1γ/CXCL12γ
|
1mg
|
-
|
说明书
|
1份
|
保存条件:
-20℃或更低温度保存,至少一年有效。由于蛋白的每次冻融均会引起部分失活,所以首次配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液),须分装后-20℃或更低温度冻存,以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用,溶液中的蛋白很容易粘附在管壁上,并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein,如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点,使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。
为了您的安全和健康,请穿实验服并戴一次性手套操作。