Recombinant Human HCC-1, 66a.a./CCL14

Name

Recombinant Human HCC-1, 66a.a./CCL14 (Recombinant Human Hemofiltrate CC Chemokine-1, 66a.a./CCL14; rHuHCC-1, 66a.a./CCL14)；重组人Hemofiltrate CC趋化因子1，66a.a./CCL14

Synonyms

CCL14; HCC-1

Purity

>95% by SDS-PAGE and HPLC analyses.

Biological Activity

Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human monocytes is in a concentration range of 5.0-20ng/ml.

Physical Appearance

Sterile Filtered lyophilized (freeze-dried) liquid-like powder.

Formulation

Lyophilized from a 0.2μm filtered concentrated solution in 2X PBS, pH7.4, 5% trehalose.

Endotoxin

Less than 1EU/μg of rHuHCC-1, 66a.a./CCL14 as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.

Category

Chemokines

Background

Human CCL14 is belonging to the CC chemokine family. It is encoded by the gene CCL14. CCL14 has two isoforms, CCL14a (HCC-1) and CCL14b (HCC-3). The sequence of HCC-3 differs from HCC-1 as follow: 27-27 R→ QTGGKPKVVKIQLKLVG. CCL14 was first isolated from the hemofiltrate of human patients with chronic renal failure. The N-terminal processed forms HCC-1(3-74), HCC-1(4-74) and HCC-1(9-74) are produced in small amounts by proteolytic cleavage after secretion in blood. CCL14 promotes chemotaxis of T lymphocytes, monocytes and eosinophils, and inhibits infection of M-tropic human immunodeficiency virus type 1 and is a ligand for CCR1, CCR3 and CCR5. Recombinant human CCL14 (66a.a.) contains 66 amino acid residues and activation of the HCC 1/CCL14a precursor to active peptide is mediated by the urokinase type plasminogen activator or plasmin.

Amino Acid Sequence

GPYHPSECCF TYTTYKIPRQ RIMDYYETNS QCSKPGIVFI TKRGHSVCTN PSDKWVQDYI KDMKEN

产品编号

产品名称

包装

KL6623-2μg

Recombinant Human HCC-1, 66a.a./CCL14

2μg

KL-6623-10μg

Recombinant Human HCC-1, 66a.a./CCL14

10μg

KL-6623-100μg

Recombinant Human HCC-1, 66a.a./CCL14

100μg

KL-6623-1mg

Recombinant Human HCC-1, 66a.a./CCL14

1mg

-

说明书

1份