产品编号 |
产品名称 |
产品包装 |
产品价格 |
P6623-2μg |
Recombinant Human HCC-1, 66a.a./CCL14 |
2μg |
464.00元 |
Species
|
Gene ID
|
Accession
|
Source
|
Length
|
MW
|
Tag
|
Human
|
6358
|
Q16627
|
E. coli
|
66aa
|
7.8kDa
|
-
|
About this Antibody
|
Name
|
Recombinant Human HCC-1, 66a.a./CCL14 (Recombinant Human Hemofiltrate CC Chemokine-1, 66a.a./CCL14; rHuHCC-1, 66a.a./CCL14);重组人Hemofiltrate CC趋化因子1,66a.a./CCL14
|
Synonyms
|
CCL14; HCC-1
|
Purity
|
>95% by SDS-PAGE and HPLC analyses.
|
Biological Activity
|
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human monocytes is in a concentration range of 5.0-20ng/ml.
|
Physical Appearance
|
Sterile Filtered lyophilized (freeze-dried) liquid-like powder.
|
Formulation
|
Lyophilized from a 0.2μm filtered concentrated solution in 2X PBS, pH7.4, 5% trehalose.
|
Endotoxin
|
Less than 1EU/μg of rHuHCC-1, 66a.a./CCL14 as determined by LAL method.
|
Reconstitution
|
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
|
Category
|
Chemokines
|
Background
|
Human CCL14 is belonging to the CC chemokine family. It is encoded by the gene CCL14. CCL14 has two isoforms, CCL14a (HCC-1) and CCL14b (HCC-3). The sequence of HCC-3 differs from HCC-1 as follow: 27-27 R→ QTGGKPKVVKIQLKLVG. CCL14 was first isolated from the hemofiltrate of human patients with chronic renal failure. The N-terminal processed forms HCC-1(3-74), HCC-1(4-74) and HCC-1(9-74) are produced in small amounts by proteolytic cleavage after secretion in blood. CCL14 promotes chemotaxis of T lymphocytes, monocytes and eosinophils, and inhibits infection of M-tropic human immunodeficiency virus type 1 and is a ligand for CCR1, CCR3 and CCR5. Recombinant human CCL14 (66a.a.) contains 66 amino acid residues and activation of the HCC 1/CCL14a precursor to active peptide is mediated by the urokinase type plasminogen activator or plasmin.
|
Amino Acid Sequence
|
GPYHPSECCF TYTTYKIPRQ RIMDYYETNS QCSKPGIVFI TKRGHSVCTN PSDKWVQDYI KDMKEN
|
包装清单:
产品编号
|
产品名称
|
包装
|
P6623-2μg
|
Recombinant Human HCC-1, 66a.a./CCL14
|
2μg
|
P6623-10μg
|
Recombinant Human HCC-1, 66a.a./CCL14
|
10μg
|
P6623-100μg
|
Recombinant Human HCC-1, 66a.a./CCL14
|
100μg
|
P6623-1mg
|
Recombinant Human HCC-1, 66a.a./CCL14
|
1mg
|
-
|
说明书
|
1份
|
保存条件:
-20℃或更低温度保存,至少一年有效。由于蛋白的每次冻融均会引起部分失活,所以首次配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液),须分装后-20℃或更低温度冻存,以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用,溶液中的蛋白很容易粘附在管壁上,并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein,如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点,使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品在冻干时添加了海藻糖作为保护剂,此时冻干后本产品看起来仍然像是液体,属于正常现象。在冻干工艺上,海藻糖作为常用的冻干保护剂,可显著阻止蛋白质二级结构的改变,防止冻干过程中的蛋白变性。
本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。
为了您的安全和健康,请穿实验服并戴一次性手套操作。