Recombinant Equine IL-2 Cys141Ser

Name

Recombinant Equine IL-2 Cys141Ser (Recombinant Equine Interleukin-2 Cys141Ser; rEqIL-2 Cys141Ser)；重组马白细胞介素2 Cys141Ser

Synonyms

aldesleukin; Aldesleukin; IL-2lymphokine; interleukin 2; interleukin-2; involved in regulation of T-cell clonal expansion; T cell growth factor; T-cell growth factor; TCGF

Purity

>97% by SDS-PAGE and HPLC analyses.

Biological Activity

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine CTLL-2 cells is less than 1.0μg/ml, corresponding to a specific activity of >1000IU/mg.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH6.5.

Endotoxin

Less than 0.1EU/μg of rEqIL-2 Cys141Ser as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.

Category

Cytokine

Background

IL-2 is a powerful immunoregulatory lymphokine produced by T-cells in response to antigenic or mitogenic stimulation. It is expressed by CD4⁺ and CD8⁺ T cells, γδ T cells, B cells, dendritic cells, and eosinophils. IL-2/IL-2R signaling is required for T cell proliferation and other fundamental functions which are essential for the immune response. The receptor for IL-2 consists of three subunits (55kDa IL2Rα, 75kDa IL2Rβ, 64kDa common gamma chain γc/IL2Rγ) that are present on the cell surface in varying preformed complexes. Recombinant equine IL-2 is a 14.9kDa protein containing 129 amino acid residues. At the amino acid sequence level, equine IL-2 shares 72%, 70%, 56% and 54% sequence similarities with human, porcine, rat and murine IL-2, respectively. It has been reported that equine IL-2 augmented proliferation in equine peripheral blood mononuclear cells, but has no effect on murine CTLL2 cells.

Amino Acid Sequence

APTSSSKRET QQQLKQLQMD LKLLLEGVNN NKNPKLSKML TFKINMPKKA TELKHLQCLE EELKPLEEML KNFLSKDIKE LMSNINVTVL GLKGSETRFT CEYDDETGTI VEFLNKWITF SQSIFSTMT

产品编号

产品名称

包装

KL-6479-2μg

Recombinant Equine IL-2 Cys141Ser

2μg

KL-6479-10μg

Recombinant Equine IL-2 Cys141Ser

10μg

KL-6479-100μg

Recombinant Equine IL-2 Cys141Ser

100μg

KL-6479-1mg

Recombinant Equine IL-2 Cys141Ser

1mg

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说明书

1份