产品编号 |
产品名称 |
产品包装 |
产品价格 |
P6479-100μg |
Recombinant Equine IL-2 Cys141Ser |
100μg |
3554.00元 |
Species
|
Gene ID
|
Accession
|
Source
|
Length
|
MW
|
Tag
|
Equine
|
100034204
|
P37997
|
E. coli
|
129aa
|
14.9kDa
|
-
|
About this Antibody
|
Name
|
Recombinant Equine IL-2 Cys141Ser (Recombinant Equine Interleukin-2 Cys141Ser; rEqIL-2 Cys141Ser);重组马白细胞介素2 Cys141Ser
|
Synonyms
|
aldesleukin; Aldesleukin; IL-2lymphokine; interleukin 2; interleukin-2; involved in regulation of T-cell clonal expansion; T cell growth factor; T-cell growth factor; TCGF
|
Purity
|
>97% by SDS-PAGE and HPLC analyses.
|
Biological Activity
|
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine CTLL-2 cells is less than 1.0μg/ml, corresponding to a specific activity of >1000IU/mg.
|
Physical Appearance
|
Sterile Filtered White lyophilized (freeze-dried) powder.
|
Formulation
|
Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH6.5.
|
Endotoxin
|
Less than 0.1EU/μg of rEqIL-2 Cys141Ser as determined by LAL method.
|
Reconstitution
|
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
|
Category
|
Cytokine
|
Background
|
IL-2 is a powerful immunoregulatory lymphokine produced by T-cells in response to antigenic or mitogenic stimulation. It is expressed by CD4+ and CD8+ T cells, γδ T cells, B cells, dendritic cells, and eosinophils. IL-2/IL-2R signaling is required for T cell proliferation and other fundamental functions which are essential for the immune response. The receptor for IL-2 consists of three subunits (55kDa IL2Rα, 75kDa IL2Rβ, 64kDa common gamma chain γc/IL2Rγ) that are present on the cell surface in varying preformed complexes. Recombinant equine IL-2 is a 14.9kDa protein containing 129 amino acid residues. At the amino acid sequence level, equine IL-2 shares 72%, 70%, 56% and 54% sequence similarities with human, porcine, rat and murine IL-2, respectively. It has been reported that equine IL-2 augmented proliferation in equine peripheral blood mononuclear cells, but has no effect on murine CTLL2 cells.
|
Amino Acid Sequence
|
APTSSSKRET QQQLKQLQMD LKLLLEGVNN NKNPKLSKML TFKINMPKKA TELKHLQCLE EELKPLEEML KNFLSKDIKE LMSNINVTVL GLKGSETRFT CEYDDETGTI VEFLNKWITF SQSIFSTMT
|
包装清单:
产品编号
|
产品名称
|
包装
|
P6479-2μg
|
Recombinant Equine IL-2 Cys141Ser
|
2μg
|
P6479-10μg
|
Recombinant Equine IL-2 Cys141Ser
|
10μg
|
P6479-100μg
|
Recombinant Equine IL-2 Cys141Ser
|
100μg
|
P6479-1mg
|
Recombinant Equine IL-2 Cys141Ser
|
1mg
|
-
|
说明书
|
1份
|
保存条件:
-20℃或更低温度保存,至少一年有效。由于蛋白的每次冻融均会引起部分失活,所以首次配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液),须分装后-20℃或更低温度冻存,以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用,溶液中的蛋白很容易粘附在管壁上,并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein,如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点,使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。
为了您的安全和健康,请穿实验服并戴一次性手套操作。